1. IPA, WebGestalt, GSEA, String
2. Not described
3. IPA: Canonical Pathways, GSEA: MSigDB, STRING: KEGG pathways and InterPro, Reactome: Reactome pathways, Webgestalt: PathwayCommons
4. Yes
5. IPA; Right‑tailed Fisher’s exact test; WebGestalt hypergeometric test
6. GSEA yes; IPA,webgestalt no