Abstract Background Exosomes carry various types of transcripts and serve as promising biomarkers for inflammatory diseases. However, the role of serum exosomal microRNAs (miRNAs) in chronic rhinosinusitis with nasal polyps (CRSwNP) is poorly clarified. Methods A prospective exploratory cohort of 10 CRSwNP patients was conducted, and the serum exosome samples were subjected to miRNA sequencing. Two independent prospective cohorts, consisting of 40 and 54 patients respectively, were recruited from different medical centers for validation. These cohorts were monitored for over two years, with postoperative recurrence serving as the primary outcome measure. The top 3 differentially exosomal miRNAs were validated in the serum samples, and their predictive values for recurrence were assessed. Results Eight CRSwNP patients completed the follow-up, comprising 4 non-recurrent cases and 4 recurrent cases. Distinctive profiles of serum exosomal miRNAs were identified between the two groups. In the first validation cohort, reverse transcription-polymerase chain reaction results indicated elevated serum exosomal miR-3174 and miR-6750-5p expressions, along with reduced miR-192-3p levels in the recurrence group compared to the non-recurrence group. Receiver operating characteristic (ROC) curves and Kaplan-Meier survival analysis demonstrated significant correlations between expressions of exosomal miR-3174 and miR-192-3p and the risk of postoperative recurrence. These findings were further validated in the second cohort, confirming the elevation of both miRNAs in the recurrence group and their associations with recurrence risk. Additionally, serum exosomal miR-3174 levels increased in recurrent cases compared to their baseline levels. Conclusion Circulating exosomal microRNA signatures may influence the risk of postoperative recurrence in CRSwNP patients. Serum exosomal elevated exosomal miR-3174 and decreased miR-192-3p were correlated with CRSwNP recurrence risk. Keywords: exosome, chronic rhinosinusitis with nasal polyps, biomarker, recurrence Introduction Chronic rhinosinusitis with nasal polyps (CRSwNP) represents a prevalent and debilitating chronic inflammatory condition in the nasal cavity and sinuses.[36]^1^,[37]^2 Despite advancements in medical therapies, and the widespread adoption of functional endoscopic sinus surgery (FESS) as the alternative surgical intervention for those patients who respond poorly to conservative treatments.[38]^3^,[39]^4 Although the apparent potential of FESS can be advantageous for most patients, the effective management of this condition is impeded by the looming risk of postoperative recurrence.[40]^5^,[41]^6 This challenge is exacerbated by the intricate etiology and pathogenic mechanisms underlying CRSwNP. The recurrence not only prompts the need for additional medical interventions and surgeries but also exerts a considerable toll on the patient’s overall quality of life.[42]^7^,[43]^8 Postoperative recurrence frequently results in the return of disease symptoms, which can sometimes be more severe, causing increased distress for patients. Moreover, for individuals who do not respond adequately to medical treatments, revision surgery remains the sole option for symptom relief.[44]^9^,[45]^10 Although several biomarkers, such as single-nucleotide polymorphisms,[46]^11 circulating metabolites,[47]^6 and circulating cytokines,[48]^12 have been associated with the recurrence of CRSwNP, none have been clinically validated or made available for routine use. Consequently, there is a critical need to delve into the identification of early predictive biomarkers for recurrent CRSwNP and to unravel the potential mechanisms underlying recurrence. Such efforts are pivotal for developing targeted treatment strategies and, ultimately, achieving the goal of individualized precision therapy. In recent years, extracellular vesicles, particularly exosomes, have become crucial facilitators of communication between cells and potential repositories of disease-specific molecular information.[49]^13–15 Among the cargo transported by exosomes, microRNAs (miRNAs) stand out as promising candidates due to their regulatory roles in gene expression and their stability in various bodily fluids, including serum.[50]^16 Exosomes carrying miRNAs are involved in the underlying mechanisms of various diseases.[51]^16^,[52]^17 Recently, accumulating evidence has indicated the considerable promise of circulating exosomal miRNAs as non-invasive biomarkers for disease prognosis and the prediction of recurrence.[53]^18^,[54]^19 This potential has been demonstrated across various chronic inflammatory conditions, encompassing osteoarthritis,[55]^20 inflammatory bowel disease,[56]^21 chronic obstructive pulmonary disease,[57]^22 and allergic rhinitis.[58]^23 However, there is currently no reported information on the expression profile and role of circulating exosomal miRNAs in CRSwNP, as well as their potential as biomarkers for predicting postoperative recurrence. This study aims to delineate the circulating exosomal miRNA profiles in CRSwNP patients through sequencing. It seeks to leverage the diagnostic and prognostic potential of serum exosomal miRNAs to identify distinctive molecular signatures linked to the risk of postoperative recurrence in CRSwNP patients, with validation conducted in two independent cohorts. The findings will contribute to the establishment of a clinically relevant, non-invasive tool that provides clinicians with valuable insights into the likelihood of postoperative recurrence, facilitating timely and targeted interventions to enhance long-term outcomes for individuals with CRSwNP. Methods Patients and Settings This prospective study consists of one discovery cohort and two independent cohorts. The discovery cohort initially recruited 10 CRSwNP patients, and the first and second validation cohorts initially included 50 and 64 CRSwNP patients treated with FESS between March 2021 and May 2021. The including criteria are as following: (1) patients meeting the diagnostic criteria for CRSwNP as defined by the European Position Paper on Rhinosinusitis and Nasal Polyps 2012;[59]^24 (2) poor response to medication necessitating FESS; (3) age between 18 and 65 years; (4) no previous history of FESS. Exclusion criteria: (1) incomplete clinical data, diagnoses of fungal sinusitis, allergic fungal rhinosinusitis, or sinonasal tumors; (2) recent use of oral corticosteroids, antibiotics within the last month; (3) concomitant acute inflammation or asthma; (4) age below 18 or above 65 years. The Ethics Committee of the Affiliated Changsha Central Hospital of Hengyang Medical School approved the research protocol (No. 2023097). All patients provided written informed consent. Follow-Up and Definition of Recurrence All CRSwNP patients underwent FESS performed by experienced surgeons, following consistent surgical standards. Postoperatively, patients received standard care, including nasal irrigation and steroid nasal spray. In cases of persistent symptoms, additional measures such as oral antibiotics and prednisone were administered as previously described.[60]^25^,[61]^26 Postoperative follow-up appointments were performed to monitor disease progression. Recurrence was defined as the persistence of disabling symptoms, endoscopic indicators, or CT findings for at least 2 months despite the prescribed antibiotic and oral steroid rescue regimen.[62]^27^,[63]^28 The minimum follow-up duration for all patients was 2 years, and they were divided into nonrecurrence and recurrence groups. Clinical Data and Serum Sample Collection Demographic and clinical data were collected from all subjects before their recruitment, including sex, age, body mass index (BMI), accompanying allergic rhinitis, asthma and aspirin intolerance, and Lund-MacKay and Lund-Kennedy scores. Blood samples from CRSwNP patients were collected using vacuum tubes containing a coagulant on the day of admission, usually 2–3 days before undergoing FESS. In the second validation cohort, blood specimens were collected from individuals who experienced recurrence, while those who did not experience recurrence provided blood samples again after a 2-year follow-up. All harvested samples were immediately centrifuged at 3000 g for 10 min to isolate serum samples. The collected serum specimens were kept at −80 °C until analysis. Serum Exosome Isolation and Identification The serum exosomes were isolated with the Total Exosome Isolation Reagent kit (Invitrogen^®, USA), and then subjected to either identification or RNA extraction. After purifying the exosomes, transmission electron microscopy (TEM) was utilized to observe their structure and morphology following previously described protocols.[64]^22 The particle size and size distribution of exosomes were analyzed by dynamic light scattering. Western bolting was performed to detect the protein expression of the specific exosome markers in both exosome samples and serum without exosome samples. The specific markers primary antibodies, including CD81 (Affinity, China) and CD63 (Affinity, China), and secondary antibodies (Affinity, China) were used. RNA Extraction and RNA-Sequencing RNA was extracted from exosomes obtained from CRSwNP patients, and the concentration and purity were assessed. Following RNA extraction, a sequencing library was prepared using the NEB Multiplex Small RNA Library Prep Set for Illumina (Illumina, USA) according to established protocols outlined in previous studies.[65]^23^,[66]^29 This process involved reverse transcription, cDNA synthesis, adapter addition, and sequencing primer ligation. Following these steps, the sequencing library was subjected to 50-cycle sequencing on the Illumina NextSeq 500 platform (Illumina, USA). Data Analysis and Target Prediction In the discovery cohort, 8 CRSwNP patients completed the follow-up, including 4 cases without recurrence and 4 cases with recurrence. The raw sequencing data received stringent quality control and subsequent analysis using the statistical R package. Differentially expressed miRNAs were identified based on a fold change (FC) ≥1.5 or FC ≤0.67 and a P-value <0.05, comparing the non-recurrence and recurrence groups. Heatmap analysis and volcano plots were generated to visually represent the differentially expressed miRNAs. Pathway enrichment analysis was conducted to discern the enriched target genes of the differentially expressed miRNAs. Validation of miRNA To corroborate the sequencing findings, we selected the three most dysregulated exosomal miRNAs for validation using reverse transcription-quantitative polymerase chain reaction (RT-PCR). During this procedure, RNA extraction from exosomes was utilized as a template for reverse transcription. The cDNA synthesis was carried out using total RNA and the Mir-X miRNA First-Strand Synthesis Kit (Takara, China) following the provided instructions. Following this, quantitative PCR was employed to amplify and quantify the cDNA molecules associated with the target miRNAs, using the miRNAs primer outlined in [67]Table S1. The relative expression levels of miRNAs were normalized to the external control cel-miR-39 as previously described.[68]^23^,[69]^29 Statistical Analysis Variables with a normal distribution are reported as mean ± SD and analyzed using Student’s t-test. For variables lacking a normal distribution, median and interquartile ranges (IQRs) are provided, and the Mann–Whitney U-test is utilized for comparisons. Categorical data are shown as frequencies and percentages, and compared with the chi-square test. Receiver operating characteristic (ROC) curves are employed to evaluate the predictive values of serum exosomal miRNAs for recurrence, the area under the curve (AUC) and 95% confidence interval (CI) were recorded to evaluate the predictive ability. CRSwNP patients are stratified into high and low-miRNA groups based on the median values of serum exosomal miRNA levels. Kaplan-Meier survival analysis is conducted to analyze their associations with the risk of postoperative recurrence. Data analysis is performed using SPSS 21.0 (SPSS, USA) and GraphPad Prism 7.0 (GraphPad, USA). A significance level of 0.05 is applied for all tests, with p-values below this threshold considered statistically significant. Results Identification of Exosomes Following a two-year follow-up, 4 CRSwNP patients were assigned to the non-recurrence group, while 4 patients were categorized into the recurrence group. TEM images displayed the typical round cup-shaped morphology of exosomes collected from both groups ([70]Figure 1A and [71]B). Nanoparticle tracking analysis results illustrated particle diameters ranging from 80 to 100 nm ([72]Figure 1C and [73]D). Additionally, WB images demonstrated significant expressions of CD81 and CD63 in the detected exosome samples, with sparse expression observed in serum samples devoid of exosomes ([74]Figure 1E). These findings collectively confirm the successful collection of exosomes from the two groups. Figure 1. [75]Figure 1 [76]Open in a new tab Identification of serum exosomes in CRSwNP patients. The representative images were observed by transmission electron microscopy in non-recurrent patients (A) and recurrent patients (B). Particle diameter analysis of exosomes in non-recurrent patients (C) and recurrent patients (D). (E) Marker protein of exosome observed by. CRSwNP, chronic rhinosinusitis with nasal polyps; WB, Western blotting. Serum Exosomal miRNA Profiles Between the Non-Recurrence and Recurrence Groups The sequencing results, as illustrated by heat maps and volcano plots in [77]Figure 2A and [78]B, unveiled distinct serum exosomal miRNA profiles distinguishing the non-recurrence and recurrence groups. A total of 818 serum exosomal miRNA were detected between the two groups. Among them, 74 miRNAs exhibited differential expression in the recurrence group compared to the non-recurrence group, with 46 being up-regulated and 28 down-regulated. To elucidate the biological significance of exosomal miRNAs in CRSwNP, we predicted potential target genes for the differentially expressed miRNAs. The KEGG pathway analysis indicated that these miRNAs were prominently involved in pathways related to bacterial invasion of epithelial cells and the TGF-beta signaling pathway ([79]Figure 2C and [80]D). Figure 2. [81]Figure 2 [82]Open in a new tab Identification of exosomal miRNA expression profiles between the non-recurrence and recurrence groups. Heatmap analysis (A) and volcano plot (B) showing differentially expressed miRNA between the non-recurrence and recurrence groups. KEGG pathway analysis of target genes of up-regulated (C) and down-regulated (D) miRNAs. KEGG, Kyoto encyclopedia of genes and genomes. Validation of Exosomal miRNA Profiles in Validation Cohorts The top 3 dysregulated serum exosomal miRNAs were selected for validation in the first validation cohort. The detailed parameters of these miRNAs are listed in [83]Table 1. This first validation cohort comprised 40 CRSwNP patients who completed a two-year follow-up, with 25 patients categorized as non-recurrent and 15 as recurrent. [84]Table 2 details the demographic and clinical data between the two groups. The RT-PCR results in [85]Figure 3 showed that serum exosomal miR-4657, miR-6750-5p, and miR-6750-5p levels were increased, while miR-192-3p levels were reduced in the recurrence group compared to the non-recurrence group. ROC curves demonstrated that serum exosomal miR-6750-5p, miR-3174, and miR-192-3p levels exhibited predictive values for CRSwNP recurrence ([86]Figure 4A–F). Additionally, Kaplan-Meier survival analysis suggested that serum exosomal miR-3174 and miR-192-3p levels were associated with the risk of postoperative recurrence ([87]Figure 4G–L). Table 1. The Parameters of Top 3 Up-Regulated and Down-Regulated miRNAs Mature ID Length FC Log2FC Regulation P value miR-4657 23 251.218 7.97 up 0.007 miR-6750-5p 24 197.561 7.63 up 0.014 miR-3174 23 169.353 7.40 up 0.021 miR-7975 18 0.002 −8.96 down 0.002 miR-520d-3p 22 0.002 −8.67 down 0.003 miR-192-3p 22 0.003 −8.52 down 0.002 [88]Open in a new tab Abbreviation: FC, fold change. Table 2. Demographic Characteristics of CRSwNP Patients in the First Validation Cohort Non-Recurrence Recurrence P Number, n 25 15 Male sex, n (%) 15 (60.0) 8 (53.3) 0.749 Age (years) 46.0 (37.0, 52.0) 49.0 (35.0, 54.0) 0.709 BMI, kg/m[89]^2 23.0 (20.8, 24.6) 22.6 (21.3, 24.4) 0.977 Allergic rhinitis, n (%) 4 (16.0) 6 (40.0) 0.135 Asthma, n (%) 2 (8.0) 4 (26.7) 0.174 Aspirin intolerance, n (%) 1 (4.0) 2 (13.3) 0.545 Lund-MacKay score 12.0 (11.0, 13.5) 12.0 (10.0, 13.0) 0.640 Lund-Kennedy score 7.0 (6.0, 8.5) 7.0 (6.0, 9.0) 0.928 [90]Open in a new tab Abbreviations: CRSwNP, chronic rhinosinusitis with nasal polyps; BMI, body mass index. Figure 3. [91]Figure 3 [92]Open in a new tab Validation of top 3 up-regulated and down-regulated expressed exosomal miRNAs in the first validation cohort. (A) miR-4657; (B) miR-6750-5p; (C) miR-3174; (D) miR-7975; (E) miR-520d-3p; (F) miR-192-3p. ns, no significance; *P<0.05, **P<0.01; ***P<0.001. Figure 4. [93]Figure 4 [94]Open in a new tab The predictive values of serum exosomal miRNAs for postoperative recurrence in the first validation cohort. (A–F) ROC curves. (G–L) Kaplan-Meier survival analysis. ROC, receiver operating characteristic. To further confirm the associations between potential serum exosomal miRNAs and postoperative recurrence, we assessed the levels of serum exosomal miR-3174 and miR-192-3p in the second validation cohort from another medical center. This cohort included 54 CRSwNP patients, with 35 non-recurrent patients and 19 recurrent patients after a two-year follow-up. [95]Table 3 provides the demographic and clinical data between the two groups. The RT-PCR results in [96]Figure 5A and [97]B revealed that miR-3174 was increased, and miR-192-3p was decreased in the serum exosomes in the recurrence group compared to the non-recurrence group. ROC and Kaplan-Meier survival analysis suggested that serum exosomal miR-3174 and miR-192-3p levels were associated with the risk of postoperative recurrence ([98]Figure 5C–F). Moreover, our findings indicated that serum exosomal miR-3174 and miR-192-3p levels were significantly enhanced when CRSwNP patients experienced postoperative recurrence compared to the baseline levels, with no significant change observed in non-recurrent cases between baseline and 2 years post-FESS levels ([99]Figure 6). These results imply that elevated serum exosomal miR-3174 may contribute to the recurrent mechanisms of CRSwNP. Table 3. Demographic Characteristics of CRSwNP Patients in the Second Validation Cohort Non-recurrence Recurrence P Number, n 35 19 Male sex, n (%) 21 (60.0) 12 (63.2) 1.000 Age (years) 42.0 (34.0, 51.0) 41.0 (25.0, 54.0) 0.537 BMI, kg/m[100]^2 23.3 (21.9, 25.7) 23.7 (21.1, 24.6) 0.384 Allergic rhinitis, n (%) 6 (17.1) 7 (36.8) 0.181 Asthma, n (%) 3 (8.6) 5 (26.3) 0.113 Aspirin intolerance, n (%) 2 (5.7) 3 (15.8) 0.332 Lund-MacKay score 12.0 (11.0, 14.0) 13.0 (11.0, 15.0) 0.356 Lund-Kennedy score 8.0 (6.0, 9.0) 7.0 (6.0, 9.0) 0.899 [101]Open in a new tab Abbreviations: CRSwNP, chronic rhinosinusitis with nasal polyps; BMI, body mass index. Figure 5. [102]Figure 5 [103]Open in a new tab Validation of serum exosomal miR-4657 and miR-192-3p in the second validation cohort. (A and B) the expressions of exosomal miR-4657 and miR-192-3p in the serum samples between the non-recurrence and recurrence groups. (C and D) ROC curves. (E and F) Kaplan-Meier survival analysis. ROC, receiver operating characteristic. *P<0.05, ***P<0.001. Figure 6. [104]Figure 6 [105]Open in a new tab Comparison of serum exosomal miR-4657 and miR-192-3p levels between baseline and recurrence time points. (A and B) Comparison of expression levels between baseline and 2 years post-FESS in non-recurrent CRSwNP patients. (C and D) Comparison of expression levels between baseline and recurrence in recurrent cases. FESS, functional endoscopic sinus surgery; CRSwNP, chronic rhinosinusitis with nasal polyps. ns, no significance; *P<0.05. Discussion The pathological mechanisms of CRSwNP are poorly clarified, and the high rate of recurrence poses a significant clinical challenge due to its high tissue heterogeneity. Exploring early predictors of recurrent CRSwNP and clarifying the underlying recurrent mechanisms are current hot research topics and challenges. Although previous studies have identified various objectively abnormal expressions of biomarkers closely associated with the risk of CRSwNP recurrence, such as serum metabolites,[106]^6 nasal microbiota,[107]^30 and tissue eosinophilia,[108]^28 the accuracy of these biomarkers needs further improvement before being applied in clinical practice. Exosomes, as standout molecules in current research, can express miRNAs in the serum.[109]^22^,[110]^31 Previous studies have confirmed that various diseases exhibit specific serum exosomal miRNA expression profiles and multiple miRNAs have been identified with potential capabilities for disease diagnosis and prognosis assessment.[111]^32^,[112]^33 Although several previous studies have emphasized the involvement of tissue circRNA/miRNA ceRNA networks in the pathological mechanisms of CRSwNP,[113]^34 there is currently a lack of research on the expression and mechanisms of exosome-carried miRNAs in CRSwNP. Despite a recent study discovering differences in the exosomal miRNA expression profile in nasal lavage fluid between chronic rhinosinusitis patients and healthy controls, the clinical significance and specific mechanisms of the associated miRNAs were not elucidated.[114]^35 Therefore, exploring the associations between serum exosomal miRNA expression profiles and postoperative recurrence in CRSwNP, and clarifying the intricate interplay between these small RNA molecules and the molecular pathways implicated in CRSwNP pathogenesis provides a unique opportunity to uncover novel insights into the mechanisms governing postoperative recurrence. In this prospective study, we first analyzed serum exosomal miRNA profiles in CRSwNP patients and found that recurrent CRSwNP patients exhibited distinctive baseline miRNA profiles in comparison with non-recurrent cases. The candidate serum exosomal miRNAs were subsequently validated in two independent cohorts from different medical centers, confirming the predictive values of serum exosomal miR-3174 and miR-192-3p for postoperative recurrence in CRSwNP. Intriguingly, miR-3174 was significantly increased in the serum exosomes of CRSwNP patients who suffered a recurrence in comparison with their baseline levels. These results suggest that serum exosomal miRNA profiles affected the risk of recurrence of CRSwNP, and miR-3174 might be involved in its recurrent mechanisms. We demonstrated that serum exosomal miR-3174 levels correlated with the risk of postoperative recurrence in CRSwNP patients, and its level change was involved in the process of recurrence. KEGG pathway analysis showed that several target genes of miR-3174 were involved in the bacterial invasion of epithelial cells and the TGF-beta signaling pathways. It is well established that epithelial cell bacterial invasion and TGF-beta signaling activation were crucial in the immunopathological mechanisms of CRSwNP and associated with the recurrence.[115]^36–38 Bacterial invasion, injury, and inflammation in nasal mucosal epithelial cells can significantly impact the barrier function of nasal mucosal epithelium, hindering its repair.[116]^39^,[117]^40 Additionally, the accumulation of TGF- beta 1 promotes tissue remodeling and epithelial-mesenchymal transition (EMT) in nasal mucosal epithelium. These pathological changes have been confirmed to be closely associated with the postoperative recurrence of CRSwNP.[118]^41^,[119]^42 Recent studies have identified miR-3174 as an oncogene capable of regulating autophagy and apoptosis in tumor cells, promoting interstitial invasion of tumor cells.[120]^43^,[121]^44 Another study has confirmed that miR-3174 can facilitate the differentiation of tumor stromal cells, mediating the occurrence of EMT in liver cancer tissues.[122]^45 Combining the current research findings with existing literature, we speculate that miR-3174 carried by extracellular vesicles in serum may influence the differentiation of stromal cells in the nasal mucosa, promoting EMT and tissue remodeling, thereby participating in the postoperative recurrence process of CRSwNP. However, the relevant regulatory mechanisms require further elucidation. Another interesting finding was that serum exosomal miR-192-3p levels were reduced in the recurrent CRSwNP patients, and its levels were negatively correlated with the risk of postoperative recurrence. Currently, existing research primarily focuses on the role of miR-192-3p in tumors, revealing that its aberrant expression levels are closely associated with tumorigenesis and biological behavior.[123]^46^,[124]^47 A recent publication demonstrated that miR-192-3p was dysregulated in the synchronous colorectal adenoma tissues and associated with tissue immune cell profile, including dendritic cells, eosinophils, and macrophages.[125]^48 Hu et al[126]^49 found that miR-192-3p could target IGF2 and regulate macrophage polarization, which contributed to the etiopathogenesis of hepatocellular carcinoma. Accordingly, the immune microenvironment changes mediated by immune cells within the mucosal tissue, including macrophage M2 polarization and eosinophil recruitment are crucial mechanisms in mediating the postoperative recurrence of CRSwNP.[127]^50^,[128]^51 Combining the results of this study with existing literature, we speculate that serum exosomal miR-192-3p may influence the functionality of immune cells in nasal polyp tissue, including the regulation of macrophage M2 polarization and eosinophil recruitment, thereby affecting tissue inflammation development and postoperative recurrence. However, specific mechanisms await further confirmation. Our study has several limitations. Firstly, it is a single-center prospective study with a limited sample size and absence of healthy controls, and the predictive values of serum exosomal miRNAs should be validated in a larger, multi-center cohort study. Secondly, we focused solely on the analysis of baseline serum exosomal miRNAs in CRSwNP patients and their predictive values for postoperative recurrence, without comparing them between CRSwNP patients and healthy controls. Thirdly, we did not undertake mechanistic studies to elucidate the role of candidate miRNAs in the recurrence mechanism of CRSwNP. It is crucial to further investigate the underlying mechanisms to understand how these miRNAs contribute to the recurrence process and to explore their potential roles in disease progression and therapeutic targeting. Conclusions In this prospective study, we confirmed that serum exosomal miRNA profiles were associated with the risk of postoperative recurrence in CRSwNP patients. The discovery-validation results highlighted that serum exosomal elevated exosomal miR-3174 and decreased miR-192-3p were correlated with CRSwNP recurrence risk, suggesting they were potential biomarkers for predicting postoperative recurrence. Funding Statement This research was supported by the Changsha Municipal Natural Science Foundation Project (No. kq2208444), the Changsha Municipal Health Commission Fund Project (No. SB2023-226). Ethics and Consent Statements This study was approved by the Ethics Committee of the Affiliated Changsha Central Hospital of Hengyang Medical School approved the research protocol (No. 2023097). All patients provided written informed consent. This study complies with the Declaration of Helsinki. Disclosure The authors declare no conflicts of interest in preparing this article. References