Abstract

Background

   Lignocellulose is the most abundant renewable bioresource on earth, and
   its biodegradation and utilization would contribute to the sustainable
   development of the global environment. Ruminiclostridium papyrosolvens,
   an anaerobic, mesophilic, and cellulolytic bacterium, produces an
   enzymatic complex known as the cellulosome. As one of the most highly
   evolved species among Ruminiclostridium-type species, R. papyrosolvens
   is particularly relevant for understanding how cellulolytic clostridia
   modulate their biomass degradation mechanisms in response to diverse
   carbon sources.

Results

   Our study investigates the transcriptional responses of
   Ruminiclostridium papyrosolvens to different carbon sources to
   understand its lignocellulose utilization. Using RNA-seq, we analyzed
   gene expression under glucose, cellobiose, xylan, cellulose, and corn
   stover, identifying distinct metabolic preferences and regulatory