Abstract: Chromatin Structure Regulation in Human Adipose-Derived Stem Cell Aging [19]Ivona Percec Ivona Percec, MD, PhD ^1University of Pennsylvania, Philadelphia, PA Find articles by [20]Ivona Percec ^1, [21]Xiaoyin Shan Xiaoyin Shan, PhD ^1University of Pennsylvania, Philadelphia, PA Find articles by [22]Xiaoyin Shan ^1, [23]Cleresa Roberts Cleresa Roberts, BS ^1University of Pennsylvania, Philadelphia, PA Find articles by [24]Cleresa Roberts ^1, [25]Yemin Lan Yemin Lan, PhD ^1University of Pennsylvania, Philadelphia, PA Find articles by [26]Yemin Lan ^1 * (BUTTON) Author information * (BUTTON) Article notes * (BUTTON) Copyright and License information ^1University of Pennsylvania, Philadelphia, PA Supplement PSTM 2017 Abstract Supplement Collection date 2017 Sep. Copyright © 2017 The Authors. Published by Wolters Kluwer Health, Inc. on behalf of The American Society of Plastic Surgeons. All rights reserved. This is an open-access article distributed under the terms of the [27]Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. [28]PMC Copyright notice PMCID: PMC5636380 __________________________________________________________________ INTRODUCTION: Aging and age-related diseases have been linked to both genetic and epigenetic changes. The chromatin structure is critical to the transmission of epigenetic information impacting transcription and genomic stability. Adult stem cells play a pivotal role in the maintenance of tissue and organ homeostasis during aging and are frequently exploited in regenerative medicine and plastic surgery. Although many studies have been focused on mechanisms of regulating chromatin structure in somatic cells, the mechanism in aging adult human stem cells is not well understood. We hypothesize that understanding the chromatin structure regulation of these stem cells will provide novel therapeutic strategies for both aesthetic and reconstructive applications. Towards this end, in this study we examine global chromatin structures of adipose-derived stem cells (ASCs) from young and old patients and compare them to those of age-matched somatic fibroblasts. METHODS: Human primary ASCs and fibroblasts were isolated from 13 and 8 donors, respectively. The chromatin structure of these cells was examined with the assay for transposase-accessible chromatin using sequencing (ATAC-seq). The data was analyzed to identify genome accessible by Tn5 transposase. Principle component analysis (PCA) was used to assays chromatin structure similarities of all the samples. The Database for Annotation, Visualization and Integrated Discovery (DAVID) was used for pathway enrichment analysis of the accessible promoter regions in the genome of young and old ASCs. RESULTS: Our data demonstrated that 1.2 % of the genome in old ASCs, 1.1% in young ASCs, 0.33% in old fibroblasts and 0.51% in young fibroblasts were accessible by Tn5. PCA results demonstrated distinctively different chromatin accessibilities between ASCs and fibroblasts and specific differences between these cells with respect to aging. DAVID pathway enrichment analysis identified several pathways, including DNA damage and repair, nonsense-mediated mRNA decay, and Wnt signaling pathway, to be more accessible in ASCs from old donors. CONCLUSION: In conclusion, our data demonstrated that genome accessibility in ASCs is overall significantly higher than in fibroblasts, consistent with their stemness phenotype. At the global level, ASCs maintain a more stable chromatin structure with advancing age compared with fibroblasts that appear more susceptible to age-related defects, consistent with our prior studies. These data support the benefits ASCs impart to regenerative medicine and will be valuable to the development of novel therapeutics. __________________________________________________________________ Articles from Plastic and Reconstructive Surgery Global Open are provided here courtesy of Wolters Kluwer Health (BUTTON) Close ACTIONS * [29]View on publisher site * [30]PDF (29.5 KB) * (BUTTON) Cite * (BUTTON) Collections * (BUTTON) Permalink PERMALINK https://pmc.ncbi.nlm (BUTTON) Copy RESOURCES (BUTTON) Similar articles (BUTTON) Cited by other articles (BUTTON) Links to NCBI Databases Cite (BUTTON) * (BUTTON) Copy * [31]Download .nbib .nbib * Format: [NLM] Add to Collections ( ) Create a new collection (*) Add to an existing collection Name your collection * ____________________ Choose a collection Unable to load your collection due to an error [32]Please try again (BUTTON) Add (BUTTON) Cancel Follow NCBI [33]NCBI on X (formerly known as Twitter) [34]NCBI on Facebook [35]NCBI on LinkedIn [36]NCBI on GitHub [37]NCBI RSS feed Connect with NLM [38]NLM on X (formerly known as Twitter) [39]NLM on Facebook [40]NLM on YouTube [41]National Library of Medicine 8600 Rockville Pike Bethesda, MD 20894 * [42]Web Policies * [43]FOIA * [44]HHS Vulnerability Disclosure * [45]Help * [46]Accessibility * [47]Careers * [48]NLM * [49]NIH * [50]HHS * [51]USA.gov (BUTTON) Back to Top References