Abstract Background Recent evidence has shown that Solute Carrier Family 39 Member 10 (SLC39A10) promoted tumor progression in several cancer types. The study intended to explore the expression and function of SLC39A10 in hepatocellular carcinoma (HCC). Methods Multiple bioinformatics analyses were used to evaluate SLC39A10 expression and potential role in HCC. Quantitative real-time polymerase chain reaction and immunohistochemistry were used to confirm SLC39A10 expression. Intro studies were performed to assess the effects of SLC39A10 on HCC cells proliferation and migration. Furthermore, flow cytometry was conducted to identify its specific function in apoptosis of HCC. Results SLC39A10 was significantly over-expressed in HCC samples from both bioinformatic databases and our cohort. Survival analyses suggested patients with high expression of SLC39A10 had poor overall survival and disease-free survival (P-value <0.01). Further, the expression of SLC39A10 was positively correlated with tumor-infiltrating lymphocytes and some immune checkpoints like CTLA4, TIM3 and TGFB1. In HCC cell lines, SLC39A10 knockdown inhibited cells proliferation and migration, but promoted apoptosis. Conclusion An increased SLC39A10 expression was found and served as an unfavorable indicator of survival in HCC. Further studies suggested SLC39A10 promotes tumor aggressiveness and may provide a novel target for HCC therapy. Keywords: Solute Carrier Family 39 Member 10, hepatocellular carcinoma, immune infiltration, prognosis, cancer aggressiveness Introduction Hepatocellular carcinoma (HCC) is a malignant tumor that ranks as the fourth leading cause of cancer-related death worldwide, being responsible for 781,631 deaths globally in 2018.[50]^1 The standard curative treatments for HCC are surgical resection, liver transplantation, and local ablation. Despite the enormous progress made in treatment approaches, the long-term survival for HCC patients remains unsatisfactory due primarily to the high rates of recurrence and metastasis.[51]^2^,[52]^3 The 5-year survival rate of HCC patients in Asia is only 18.1%, lower than that in North America and Europe.[53]^4 Recent studies have provided novel insights into the biology and choice for personalized treatments of HCC.[54]^5 Tumor-specific cytotoxic T cells in HCC express immune checkpoint inhibitors, such as CTLA4, PD-L1, and TIM3, which prevent T cells from effectively recognizing tumor cells, leading to immune escape.[55]^6 The combination of bevacizumab and atezolizumab can improve median overall survival by more than 17 months and has been approved as the first-line therapy for HCC.[56]^7 Nevertheless, more than 75% of HCC patients show unsatisfactory responses to immunotherapies for reasons that remain unclear.[57]^8 Consequently, a better understanding of the molecular mechanisms involved in the regulation of HCC development and progression, as well as the associated immune microenvironment, is needed to help identify predictive biomarkers and improve survival for HCC patients. Zinc, an important trace element, is indispensable for many cellular processes, including gene expression, immune functions, meiosis, cell-cycle progression, and apoptosis.[58]^9–11 Zinc has also been reported to play a key role as a second messenger in signaling pathways related to various physiological processes.[59]^12 Because of its involvement in cell apoptosis and proliferation, the role of zinc in cancer has long been the subject of investigation. Zinc distribution is tightly regulated through the activity of two zinc transporter families, namely, solute carrier family 39 (SLC39/ZIP), which functions in zinc influx into the cytoplasm, and solute carrier family 30 (SLC30/ZnT), which facilitates zinc efflux from the cytoplasm.[60]^13 Some SLC39 family members, such as SLC39A1 and SLC39A6, are reported to be involved in cancer development and progression.[61]^14^,[62]^15 SLC39A10, one of the 14 known human SLC39 family members, can serve as a biomarker in several cancer types, including renal cell carcinoma (RCC), gastric cancer, and breast cancer.[63]^16–18 However, whether it also has a role in HCC is unknown. In our study, we assessed SLC39A10 expression and its value in predicting survival for HCC patients. We further performed bioinformatics analysis and in vitro experiments to assess how SLC39A10 affects tumor cell behavior and identify the potential underlying mechanisms. Methods Data Acquisition Gene expression data for 366 HCC samples were obtained from The Cancer Genome Atlas (TCGA) database up to April 2021 ([64]https://portal.gdc.cancer.gov/repository). The [65]GSE25097 dataset based on the [66]GPL10687 platform (contains 289 liver and 268 HCC samples), the [67]GSE36376 dataset based on the [68]GPL10558 platform (contains 193 liver and 240 HCC samples), and the [69]GSE45436 dataset based on the [70]GPL570 platform (includes 65 liver and 69 HCC samples) were downloaded from the Gene Expression Omnibus (GEO) database for expression validation ([71]https://www.ncbi.nlm.nih.gov/geo/). Gene Expression Profiling Interactive Analysis (GEPIA, [72]http://gepia.cancer-pku.cn/index.html) and Oncomine ([73]https://www.oncomine.org) were also used to validate the transcriptional level of SLC39A10 in HCC and normal liver tissues.[74]^19^,[75]^20 Tissue Samples and Patient Follow-Up For validation, tumor and adjacent normal tissue samples were obtained from 95 HCC patients at Guangdong Provincial People’s Hospital (GDPH cohort). All patients underwent surgical resection and were followed up until December 2020. Overall survival (OS) was defined as the date from surgical resection to death or last contact and disease-free survival (DFS) as the date from surgical resection to tumor metastasis or recurrence. The median follow-up time of patients from the GDPH cohort was 25.5 months (range, 16–78). The clinicopathologic information of the enrolled patients, including gender, age, hepatitis B surface antigen (HBsAg), tumor size, alpha-fetoprotein (AFP), TNM stage, and differentiation were manually collected. The study was approved by the Ethics Association of Guangdong Provincial People’s Hospital and all the enrolled patients provided written informed consent before participation. Each tissue sample was evaluated and diagnosed as HCC by two different professional pathologists. Cox Regression and Kaplan–Meier Survival Analysis Univariate and multivariate Cox regression analyses were used to identify independent prognostic factors for HCC prognosis in the GDPH cohort. Kaplan–Meier analyses and Log rank tests were conducted for the high- and low-SLC39A10 expression groups in both TCGA and GDPH cohorts to assess the ability to predict patient survival. Functional and Pathway Enrichment Analysis The GeneMANIA tool was used to analyze the relationship between SLC39A10 and its neighboring genes and construct a gene network map ([76]http://genemania.org/).[77]^21 The STRING database was used to construct a protein–protein interaction (PPI) network for SLC39A10 ([78]https://string-db.org/cgi/).[79]^22 Co-expressed genes (|Spearman correlation coefficient| >0.5 and P < 0.05) were screened from the cBioPortal database ([80]https://www.cbioportal.org/) and then integrated into DAVID 6.7 for Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses ([81]https://david-d.ncifcrf.gov/). The results of the GO and KEGG analyses were visualized using the R “ggplot2” package. Gene set enrichment analysis (GSEA) was carried out to determine the gene sets and functional pathways that differed significantly between the high- and low-SLC39A10 expression groups. SLC39A10 expression was used as a phenotype label and 1000 gene set permutations were performed per analysis. Immune Infiltration Analyses Through Multiple Datasets The Tumor Immune Estimation Resource (TIMER; [82]https://cistrome.shinyapps.io/timer/) webserver was first used to assess the relationship between SLC39A10 expression and infiltration levels of immune cells, including CD8^+ T cells, CD4^+ T cells, B cells, macrophages, dendritic cells, and neutrophils in HCC.[83]^23 Analyses of the correlation between SLC39A10 expression and gene markers of immune-infiltrating cells were conducted using Spearman correlation coefficients. The TISIDB database was subsequently used to identify putative correlations between SLC39A10 expression and immunostimulators or immunoinhibitors in HCC ([84]http://cis.hku.hk/TISIDB).[85]^24 Cell Culture and Transfection Normal human liver (LO2) and HCC (Hep3b, HUH7, PLC/PRE5, HepG2, LM3, and MHCC-97H) cell lines were obtained from Procell (Wuhan, China). Cells were cultured in RPMI 1640 medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS) at 37°C with 5% CO[2]. The vector for SLC39A10 knockdown was purchased from Obio Technology (Shanghai, China). The GV112 lentiviral vector was transfected into 293T cells using Helper 1.0 Packaging Plasmid Mix and virus particles were collected with lentivirus after 3 days. Precipitation was carried out according to the packaging scheme of System Biosciences. Cells were infected using Trans virus transduction reagent. The non-targeting (negative control, NC) plasmid was constructed with the following target sequence: GV112-NC-1CCGGTTCTCCGAACGTGTCACGTTTCAAGAGAACGTGACACGTTCGGAGAATTTTTG. cDNA for human SLC39A10 was cloned into the hU6-MCS-CMV-puro lentiviral vector and puromycin was used to screen for vector-positive cells. Cell Proliferation Assays Cell counting Kit-8 (CCK-8) and colony formation assays were used for the determination of cell viability. For CCK-8 assays, 1500 cells were seeded per well of 96-well plates. At a specific time point, CCK-8 solution was added to each well. The cells were then cultured at 37°C with 5% CO[2] and the absorbance (OD[450]) was assessed in a microplate reader after 0, 24, 48, and 72 h. For colony formation assays, cells of each cell type (2000 per well) were seeded into 6-well plates, gently shaken, and cultured at 37°C with 5% CO[2] for 7–14 days. The medium was subsequently removed and the cells were stained with 0.1% crystal violet to quantify positive colonies (diameter >30 µm). All data represent means ± SD from independent experiments performed in triplicate. Cell Migration Assays Transwell plates (Corning Costar, USA) were used for cell migration analysis. A total of 5×10^4 cells were seeded in the upper chambers of transwell plates in 200 µL of serum-free Dulbecco’s modified Eagle’s medium (DMEM) while DMEM containing 10% FBS was added to the lower chambers. After incubating for 24 h, migrated cells in the lower chambers were fixed in methanol and then stained with crystal violet. Migrated cells were imaged using an inverted microscope and quantified from three different fields. Cell migration was also evaluated by wound-healing assay. Cells (1×10^6) were seeded into each well of a 6-well plate until 80–90% confluence. A sterile 200-µL pipette tip was then used to wound the cell monolayer, following which the cells were washed twice with phosphate buffer saline (PBS). Images of the wounds were obtained at 0 and 30 h using a photomicroscope and wound closure was evaluated in at least 3 different fields using ImageJ 1.52 (National Institute of Health, USA). Flow Cytometry Flow cytometry was performed as previously described.[86]^25 Immunohistochemistry Paraffin-embedded HCC tissues were consecutively cut into 4-μm slices and then mounted on glass slides. The sections were dewaxed by soaking in dewaxing agent and 95% alcohol for 10 min and 5 min, respectively, and then washed three times with Tris-buffered saline (TBS). Antigen retrieval was performed in a high-pressure steam sterilizer (800–1200 W) using EDTA antigen-repair solution (pH 9.0). After soaking in TBS and H[2]O[2] and blocking with 10% goat serum in TBS with Tween 20 (TBST) at 25°C for 30 min, the sections were incubated with a primary antibody (diluted 1:50) against SLC39A10 (ab83947; Abcam) overnight at 4°C. The following day, the sections were soaked three times in TBST at 25°C, incubated with an anti-rabbit secondary antibody at 37°C for 45 min, washed, stained with hematoxylin, and sealed with a sealing agent. After drying, the sections were examined and photographed under an Olympus BX63 microscope. SLC39A10 immunoreactivity was determined by staining intensity and distribution; less than 25% staining was considered low expression, while ≥25% staining was considered high expression. The specimens were assessed independently by two pathologists. Real-Time Reverse Transcription Quantitative PCR (qPCR) and Western Blot qPCR and Western blotting were performed as previously described.[87]^25 The following primer pairs were used for qPCR: SLC39A10 forward 5′-TTTCACTCACATAACCACCAGC-3′ and reverse 5′-GTGATGACGTAGGCGGTGATT-3′; BCL2 forward 5′-GGTGGGGTCATGTGTGTGG-3′ and reverse 5′-CGGTTCAGGTACTCAGTCATCC-3′; BIRC5 forward 5′-AGGACCACCGCATCTCTACAT-3′ and reverse 5′-AAGTCTGGCTCGTTCTCAGTG-3′. GAPDH (reference control) forward 5′-GGTGTGAACCATGAGAAGTATGA-3′ and reverse 5′-GAGTCCTTCCACGATACCAAAG-3′. The antibodies used for Western blotting (SLC39A10 [ab83947], BCL-2 [ab32124], survivin [ab76424], and GAPDH [ab8245]) were purchased from Abcam. Statistical Analysis All statistical analyses were performed using R software version 4.0.1 ([88]https://www.r-project.org/) and SPSS version 24.0 (SPSS, Inc., Chicago, IL, USA). A P-value <0.05 was considered statistically significant unless otherwise specified. Results SLC39A10 is Highly Expressed in HCC We first obtained gene expression data for SLC39A10 in HCC patient samples from public databases as well as the GDPH cohort to evaluate the role of SLC39A10 in HCC. Data acquired from the GEPIA database showed that SLC39A10 expression was higher in HCC samples than in normal liver samples (P < 0.05) ([89]Figure 1A). Similar results were found in two datasets of the Oncomine database (Wurmbach’s and Chen’s datasets) and three GEO datasets ([90]GSE25097, [91]GSE36376, and [92]GSE45436) ([93]Figure 1B and [94]C). qPCR analysis of 20 fresh HCC and adjacent normal liver tissue samples from the GDPH cohort indicated that SLC39A10 mRNA levels were higher in tumor tissues than in control tissues ([95]Figure 1D). As expected, the immunohistochemistry (IHC) results also showed that patients with HCC displayed higher SLC39A10 expression levels than the controls (70 of the 95 HCC patients exhibited higher SLC39A10 expression while the other 25 exhibited lower SLC39A10 expression) ([96]Figure 1E and [97]F). Figure 1. [98]Figure 1 [99]Open in a new tab SLC39A10 is over-expressed in Hepatocellular Carcinoma (HCC). (A) Over-expression of SLC39A10 in HCC was identified in GEPIA database. (B) Over-expression of SLC39A10 was identified in HCC in Oncomine database (Wurmbach’s Dataset and Chen’s Dataset). (C) Over-expression of SLC39A10 was identified in HCC in three individual GEO datasets ([100]GSE25097, [101]GSE36376 and [102]GSE45436). (D) The mRNA over-expression levels of SLC39A10 were identified in HCC tissues and normal liver tissues of 20 samples. (E) Representative images of SLC39A10 staining in HCC specimens and normal liver tissues. (F) Immunohistochemistry staining showed SLC39A10 was upregulated in 70 out of 95 HCC tissues. All *P-value <0.05, **P-value <0.01, ***P-value <0.001. Abbreviations: T, tumor; N, normal. High SLC39A10 Expression Correlates with Unfavorable Clinical Characteristics and Poor Survival in HCC Patients IHC staining was performed on 95 pairs of HCC and adjacent normal tissues to evaluate the clinical value of SLC39A10. The correlation between SLC39A10 expression and the clinicopathological characteristics of HCC patients is shown in [103]Table 1 and [104]Figure 2A. High SLC39A10 expression in HCC patients was significantly correlated with poor differentiation (P < 0.05), advanced TNM stage (P < 0.05), and larger tumor size (P = 0.01). Univariate and multivariate Cox regression analyses were then performed to confirm the prognostic significance of SLC39A10. The results indicated that high SLC39A10 expression was an independent risk factor for OS and DFS among the 95 HCC patients from the GDPH cohort ([105]Tables 2 and [106]3 and [107]Figure 2B and [108]C). Additionally, Kaplan–Meier survival analyses for both TCGA and GDPH cohorts indicated that HCC patients with high SLC39A10 expression had inferior OS (P < 0.01) and DFS (P < 0.01) compared to those with low SLC39A10 expression ([109]Figure 2D–[110]G). These findings imply that SLC39A10 may be a potential indicator of HCC patient prognosis. Table 1. Correlation Between SLC39A10 Expression with Clinicopathological Characteristics of HCC Patients Clinicopathological Variables Patients (n=95) SLC39A10 Expression P value Low (25) High (70) Gender Male 83 20 63 0.29 Female 12 5 7 Age ≧50 45 11 34 0.87 <50 50 14 36 AFP ≧200 ng/mL 40 8 32 0.34 <200 ng/mL 55 17 38 HBsAg Positive 34 6 28 0.23 Negative 61 19 42 Tumor size ≧5 cm 50 19 31 0.01 <5 cm 45 6 39 TNM stage Advanced (III & IV) 39 5 32 <0.05 Early (I & II) 58 20 38 Differentiation Poor 50 8 42 <0.05 Well 45 17 28 [111]Open in a new tab Figure 2. [112]Figure 2 [113]Open in a new tab Over-expression of SLC39A10 correlates with poor survival and unfavorable clinical characteristics in Hepatocellular Carcinoma (HCC). (A) SLC39A10 expression of HCC was significantly correlated with tumor size, TNM stage and differentiation. (B-C) Multivariate Cox regression analyses were performed to confirmed high expression of SLC39A10 was independent risk factor for overall survival (OS) (B) and disease-free survival (DFS) (C) of 95 HCC patients from GDPH cohort. (D and E) Kaplan-Meier analyses showed HCC patients with high expression of SLC39A10 had inferior OS (D) and DFS (E) than those with low expression in TCGA cohort. (F and G) Kaplan-Meier analyses showed HCC patients with high expression of SLC39A10 had inferior OS (F) and DFS (G) than those with low expression in GDPH cohort. All *P-value <0.05, **P-value <0.01. Table 2. Univariate and Multivariate Cox Regression Analysis of Risk Factors Associated with Overall Survival Clinicopathological Variables Univariate Analysis Multivariate Analysis HR 95% CI P value HR 95% CI P value SLC39A10 expression (High vs Low) 8.02 5.39–8.32 <0.01 3.89 1.95–5.83 <0.01 Gender (Male vs Female) 2.02 1.23–2.81 <0.05 1.42 0.69–2.85 0.32 Age (≧50 vs <50) 1.37 0.41–2.33 0.59 AFP (≧200 ng/mL vs <200 ng/mL) 1.48 0.94–2.02 0.07 HBsAg (Positive vs Negative) 1.21 0.63–1.79 0.30 Tumor size (≧5 cm vs <5 cm) 3.56 2.42–4.7 <0.01 2.12 1.41–2.83 <0.05 TNM stage (Advanced vs Early) 3.98 3.21–4.75 <0.01 2.86 2.23–3.49 <0.01 Differentiation (Poor vs Well) 1.98 1.21–2.75 <0.05 1.19 0.70–1.68 0.21 [114]Open in a new tab Table 3. Univariate and Multivariate Cox Regression Analysis of Risk Factors Associated with Disease-Free Survival Clinicopathological Variables Univariate Analysis Multivariate Analysis HR 95% CI P value HR 95% CI P value SLC39A10 expression (High vs Low) 6.31 4.30–8.32 <0.01 3.21 2.01–4.41 <0.01 Gender (Male vs Female) 2.32 1.33–2.31 <0.01 1.23 0.34–2.12 0.45 Age (≧50 vs <50) 1.06 0.25–1.87 0.63 AFP (≧200 ng/mL vs <200 ng/mL) 1.34 0.91–1.77 0.09 HBsAg (Positive vs Negative) 1.12 0.59–1.65 0.13 Tumor size (≧5 cm vs <5 cm) 3.21 2.35–4.07 <0.01 1.65 0.48–2.82 0.31 TNM stage (Advanced vs Early) 4.34 2.98–5.70 <0.01 2.52 1.32–3.72 <0.05 Differentiation (Poor vs Well) 2.15 1.29–3.01 <0.05 1.51 0.79–2.23 0.54 [115]Open in a new tab Neighbor Gene Network and Functional Enrichment Analysis of SLC39A10 in HCC To explore the neighboring genes of SLC39A10, we first analyzed the relationships between these genes and built a gene network map using the GeneMANIA tool ([116]Figure 3A). The central node (SLC39A10) was surrounded by 20 nodes representing genes with close correlations, including SLC39A1–14, CAMK4, DHRS9, AGA, STIM2, CFAP20, NAPG, and AC006538.4. Using STRING tools, we constructed a PPI network for SLC39A10 ([117]Figure 3B) and found that SLC39A10 was connected with SLC39A2, SLC39A9, SLC39A11, SLC30A1, SLC30A4–7, and SLC30A9. Figure 3. [118]Figure 3 [119]Open in a new tab Neighbor gene network and functional enrichment analyses of SLC39A10 in Hepatocellular Carcinoma (HCC). (A) GeneMANIA tool was used to show the relationships of neighbor genes of SLC39A10 and construct a network map. (B) STRING tool was used to construct a protein-protein interaction network (PPI) of SLC39A10. (C) Gene Ontology (GO) enrichment analysis of SLC39A10. (D) Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of SLC39A10. (E) Gene set enrichment analysis (GSEA) of SLC39A10. We subsequently analyzed the potential biological pathways associated with SLC39A10 to evaluate its molecular function in HCC. A total of 89 co-expressed genes were identified using the cBioPortal dataset, and these were then enrolled into DAVID 6.7 and subjected to GO and KEGG analyses. In GO analysis, SLC39A10 and its neighboring genes were mainly enriched in “signal transduction, response to hypoxia, glucagon and drug, epidermal growth factor receptor signaling pathway, oxidation-reduction process, cell adhesion mediated by integrin, apoptotic cell clearance, peroxisome, mitochondrion, membrane, focal adhesion, cytoskeleton, SH3 domain binding, protein binding, protein kinase binding, and cytoskeletal adaptor activity” ([120]Figure 3C). KEGG pathway enrichment analysis showed that SLC39A10 may be involved in “Peroxisome, Proteoglycans in cancer, Regulation of actin cytoskeleton, and Metabolic pathways” ([121]Figure 3D). GSEA was also performed to identify the differentially enriched gene sets and pathways between HCC samples with high and low SLC39A10 expression. The top 5 significantly enriched biological processes were found to include ‘Pathways in cancer, mTOR signal pathway, PPAR signal pathway, NOTCH signal pathway, and Drug metabolism - cytochrome P450ʹ ([122]Figure 3E). These results suggested that SLC39A10 may be involved in promoting HCC tumorigenesis and progression via several signaling pathways. The Association Between Immune Infiltration and SLC39A10 Expression in HCC Tumor-infiltrating lymphocytes (TILs), especially CD8^+ T cells, are highly correlated with HCC tumorigenesis and progression.[123]^26 Here, we evaluated the correlation between SLC39A10 expression and TILs, including CD8^+ T cells, CD4^+ T cells, B cells, macrophages, dendritic cells, and neutrophils in HCC using the TIMER web server ([124]Figure 4A). After quality control, the results showed that SLC39A10 expression was positively correlated with the abundance of B cells (coefficient = 0.336, P = 1.53e-10), CD8^+ T cells (coefficient = 0.218, P = 4.64e-05), CD4^+ T cells (coefficient = 0.507, P = 7.37e-24), macrophages (coefficient = 0.492, P = 3.11e-22), neutrophils (coefficient = 0.495, P = 1.09e-22), and dendritic cells (coefficient = 0.415, P = 1.34e-15). In addition, SLC39A10 expression showed positive correlations with gene markers for T cells (CD2 and CD3E), Th1 cells (STAT4), Th2 cells (GATA3), M1 macrophages (CD80 and CD86), and other immune checkpoint markers such as CTLA4 and TIM3 ([125]Figure 4B). Figure 4. [126]Figure 4 [127]Open in a new tab Correlations of SLC39A10 expression with tumor-infiltrating lymphocytes (TILs) in Hepatocellular Carcinoma (HCC). (A) Correlations between SLC39A10 expression and TILs shown by Timer web server. (B) Correlations between SLC39A10 expression and gene markers from TILs and other immune checkpoints. To further explore the role of SLC39A10 in immune regulation, we next determined Spearman correlation coefficients for the association between SLC39A10 and immunostimulators or immunoinhibitors using the TISIDB database ([128]Figure 5A and [129]B). For immunostimulators, SLC39A10 was most negatively correlated with ICOSLG (coefficient = −0.201, P = 9.61e-05), PVR (coefficient = −0.14, P = 6.75e-03), and ENTPD1 (coefficient = −0.107, P = 0.0395) ([130]Figure 5C). For immunoinhibitors, SLC39A10 was most positively correlated with TGFB1 (coefficient = 0.364, P = 5.61e-13), LGALS9 (coefficient = 0.309, P = 1.3e-09), and HAVCR2 (coefficient = 0.191, P = 2.15e-04) ([131]Figure 5D). Combined, these results indicated that SLC39A10 may be involved in mediating tumor-specific immune responses via the regulation of TILs and several immune-related molecules. Figure 5. [132]Figure 5 [133]Open in a new tab Correlations of SLC39A10 with immunomodulators in Hepatocellular Carcinoma (HCC). (A) Correlations between immunostimulators and SLC39A10 expression shown by TISIDB database. (B) Top 3 immunostimulators with greatest negative Spearman correlation with SLC39A10. (C) Correlations between immunoinhibitors and SLC39A10 expression shown by TISIDB database. (D) Top 3 immunoinhibitors with greatest positive Spearman correlation with SLC39A10. SLC39A10 Promotes HCC Cell Proliferation and Migration SLC39A10 expression was evaluated in several HCC cell lines, with the results suggesting that it was relatively highly expressed in Hep3b and HepG2 cells ([134]Figure 6A). Three sh-SLC39A10 constructs were transfected into Hep3b and HepG2 cells to assess the effect of SLC39A10 on HCC cell proliferation and migration. qPCR results validated the efficiency of SLC39A10 knockdown in these two cell lines, especially that for Hep3b-sh-RNA2 and HepG2-sh-RNA2 (P < 0.001) ([135]Figure 6B). CCK-8 and colony formation assays both showed that, compared with the NCs, the knockdown of SLC39A10 significantly inhibited tumor cell proliferation in both Hep3b and HepG2 cells (P < 0.001) ([136]Figure 6C and [137]D). Additionally, the results of the transwell and wound-healing assays demonstrated that the migratory ability of HCC cells was suppressed with the silencing of SLC39A10 (P < 0.001) ([138]Figure 6E and [139]F). Figure 6. [140]Figure 6 [141]Open in a new tab SLC39A10 promotes the proliferation and migration of Hepatocellular Carcinoma (HCC) cells. (A) Western blot showed SLC39A10 was highly expressed in Hep3b and HepG2 cells among HCC cell lines. (B) Quantitative real-time polymerase chain reaction and Western blot were used to detect the down-regulation of SLC39A10 in Hep3b and HepG2 cells transfection with three SLC39A10-shRNA. (C and D) CCK-8 assay (C) and colony formation assay (D) showed that SLC39A10 knockdown reduced the proliferation in Hep3b and HepG2 cells. (E and F) Transwell assay (E) and wound-healing assay (F) showed that SLC39A10 knockdown reduced the migration in Hep3b and HepG2 cells. All **P-value <0.01, ***P-value <0.001. SLC39A10 Knockdown Promotes the Apoptosis of HCC Cells As our GO analysis suggested that SLC39A10 may be enriched in “Apoptotic cell clearance”, we, therefore, undertook a flow cytometric analysis to determine the specific role of SLC39A10 in HCC cell apoptosis. We found that, compared with controls, the knockdown of SLC39A10 increased the percentage of apoptotic Hep3b and HepG2 cells ([142]Figure 7A and [143]B). The results further showed that SLC39A10 expression was positively correlated BCL2 (coefficient = 0.45, P = 2.1e-19) and BIRC5 (coefficient = 0.33, P = 1.4e-10) based on GEPIA data ([144]Figure 7C). The qPCR and Western blot results confirmed that the mRNA and protein expression levels of BCL2 and BIRC5 (survivin) were downregulated in sh-SLC39A10-expressing Hep3b cells ([145]Figure 7D and [146]E). These results indicated that SLC39A10 can promote resistance to apoptosis in HCC by upregulating BCL2 and BIRC5 expression levels. Figure 7. [147]Figure 7 [148]Open in a new tab Knockdown of SLC39A10 promotes apoptosis of Hepatocellular Carcinoma (HCC) cells. (A and B) Flow cytometry showed that knockdown of SLC39A10 increased the percentage of apoptotic Hep3b (A) and HepG2 (B) cells. (C) Correlations of SLC39A10 expression and BCL2, BIRC5 shown by GEPIA web server. (D and E) Quantitative real-time polymerase chain reaction (D) and Western blot (E) validated the expression of BCL2 and BIRC5 (Survivin) was down-regulated in sh-SLC39A10 Hep3b cell. All ***P-value <0.001. Discussion Finding novel effective biomarkers for HCC initiation and progression is of great value for identifying novel therapeutic targets. Studies have shown that zinc distribution is correlated with the tumorigenesis and progression of several cancers, including hepatocarcinoma.[149]^27 Various zinc transporters in the SLC39A family have been found to mediate the dysregulated zinc distribution seen in cancers and to be involved in cancer metastasis, such as the lymph node metastasis of breast cancers.[150]^28–30 SLC39A10, a member of the SLC39A family, is a major zinc transporter involved in zinc influx and serves as a biomarker in several cancer types.[151]^16–18 Studies have shown that SLC39A10 overexpression promotes migration and invasion in breast cancer cells and is associated with the expression levels of the estrogen receptor, ERBB3, and STAT3 in breast cancer.[152]^16^,[153]^31 Additionally, SLC39A10 expression was reported to be significantly upregulated in RCC, especially in high-grade tumors.[154]^17 However, whether SLC39A10 plays a role in HCC and whether it may serve as a prognostic marker for this malignancy was not known. In the present study, we noted that SLC39A10 was highly expressed in HCC tissues both in our GDPH cohort and as determined from data available in public databases. High SLC39A10 expression was positively correlated with advanced TNM stage, poor differentiation, and large tumor size, indicating that SLC39A10 may have prognostic value in HCC. Kaplan–Meier analyses also suggested that patients with high SLC39A10 expression had poor survival. Studies have shown that SLC39A10 serves as a positive regulator of CD45R in B-cell antigen receptor signaling transduction, thereby setting a threshold in human immune responses.[155]^32 Downstream of the JAK/STAT signaling pathway, SLC39A10 mediates zinc homeostasis for early B-cell survival, thereby affecting humoral immune system maintenance.[156]^33 Using multiple tools, we explored the relationship between SLC39A10 expression and the levels of immune cell infiltration in HCC. The results showed that SLC39A10 expression was positively correlated with the abundance of TILs, including CD8^+ T cells, CD4^+ T cells, B cells, macrophages, dendritic cells, and neutrophils. However, SLC39A10 expression was also found to be positively correlated with immune checkpoint molecules such as CTLA4, TIM3, and TGFB1. Immune evasion is a hallmark of most cancers, including HCC, and is widely studied as an important therapeutic target.[157]^34 Immune checkpoint factors, including CTLA-4, PD-1/PD-L1, TIM3, and TGFB1, serve as immunosuppressors that inhibit the activities of TILs, such as natural killer cells and CD8^+ T cells, thereby promoting immune evasion in HCC.[158]^35–37 These results indicate that, although high TIL infiltration levels were found in the high-SLC39A10-expression group, SLC39A10 upregulation may simultaneously lead to increased expression of other immune checkpoint factors such as CTLA4, TIM3, and TGFB1, which consequently promote immune evasion in the HCC microenvironment. Further studies are needed to determine the relationship between SLC39A10 and TILs in the HCC immune microenvironment as well as the underlying mechanism. We constructed neighbor gene and PPI networks for SLC39A10 and identified neighbor genes that may play vital roles in cancer progression in combination with SLC39A10. This included STIM1, an important sensor molecule involved in the regulation of cytosolic Ca^2+ levels in the maintenance of B-cell functions.[159]^38 Our findings also revealed that SLC39A10 expression was positively correlated with the abundance of B cells and other immune checkpoint factors in HCC. Additional studies are needed to reveal their role in B-cell function or the tumor immune microenvironment. GO and KEGG functional enrichment analyses suggested that SLC39A10 was mainly associated with “Cell adhesion, Apoptotic cell clearance, Focal adhesion, Cytoskeleton, SH3 domain binding, Protein binding, Protein kinase binding, Cytoskeletal adaptor activity, and Regulation of actin cytoskeleton”. GSEA analysis also showed that SLC39A10 was highly associated with “Pathways in cancer, mTOR signal pathway, PPAR signal pathway, NOTCH signaling pathway, and Drug metabolism - cytochrome P450”. Given our initial findings, we further evaluated the effects of SLC39A10 on the proliferative and migratory ability of HCC cells. Consistent with the results of previous studies,[160]^16^,[161]^31 the knockdown of SLC39A10 significantly inhibited proliferation and migration in two HCC cell lines. SLC39A10 may play an antiapoptotic role in coordination with BCL members and is a critical inhibitor of caspase-mediated apoptosis.[162]^33 Driven by STAT3, SLC39A10 was also found to interact with SLC39A6 to initiate cell rounding and import zinc into cells to initiate mitosis.[163]^39 In the current study, flow cytometry results demonstrated that SLC39A10 knockdown promoted the apoptosis of HCC cells. Furthermore, the mRNA and protein expression of two important apoptosis inhibitors, BCL-2 and BIRC5/survivin, were proportionally reduced in HCC SLC39A10-knockdown cells. These observations suggest that SLC39A10 may promote resistance to apoptosis in combination with BCL-2 and BIRC5. This is the first study to evaluate and report the expression and effects of SLC39A10 in HCC. However, our study had several limitations. For example, although our results indicated that SLC39A10 may serve as a prognostic biomarker for HCC, further in vivo experiments and prospective studies are needed to validate our results and further reveal the underlying molecular mechanisms. Conclusion In summary, we found that high SLC39A10 expression is an unfavorable indicator of HCC prognosis. In vitro studies suggested that SLC39A10 is involved in the regulation of HCC cell proliferation, migration, and apoptosis. Overall, our study has provided novel ideas concerning the mechanisms underlying HCC tumorigenesis and progression, and identified a potential biomarker for HCC prognosis, as well as a potential therapeutic target for HCC treatment. Acknowledgments