Graphical abstract [41]graphic file with name ga1.jpg [42]Open in a new tab Keywords: Psoriasis, Drug repurposing, Gene expression and reversal, CMap, Drug perturbagen Abstract Psoriasis is a skin disease which results in scales on the skin caused by flaky patches. Psoriasis is triggered by various conditions such as drug reactions, trauma, and skin infection etc. Globally, there are 125 million people affected by psoriasis and yet there is no effective treatment available, and it emphasizes the need for discovery of efficacious treatments. De-novo drug development takes 10–17 years and $2–$3 billion of investment with <10 % success rate to bring drug from concept to a market ready product. A possible alternative is drug repurposing, which aims at finding other indications of already approved drugs. In this study, a computational drug repurposing framework is developed and applied to differential gene expressions of Psoriasis targets obtained from the publicly available database (GEO). This strategy uses the gene expression signatures of the Psoriasis and compares it with perturbagen available in the CMap. Based on the connected signature drugs are ranked which could possibly reverse the signatures to stop the psoriasis. The drugs with most negative connectivity scores are ranked efficient and vice versa. The top hit drugs are verified using the literature survey of the peer reviewed journal, electronic health records, patents, and hospital database. As a result, 50/150 and 37/150 drugs are confirmed to have anti-psoriasis efficacy in two datasets. Top 10 drugs are suggested as potential repurposable drugs for psoriasis. This study offers, a powerful yet simple approach for rapid identification of potential drug repurposing candidates in Psoriasis and any disease of interest. 1. Introduction Psoriasis is a common autoimmune disease that results in flaky patches of skin which form scales. It mostly appears on the knees, elbows, and scalp. The autoimmune disorder is linked with hyperproliferation of skin cells and normally known by red itchy spots, thick and scaly skin, and lesions appearing from hyperkeratosis [43][1]. In psoriatic disease conditions, crosstalk between immune cells and keratinocytes is mediated by the release of cytokines, chemokines, and growth factors. Patients experiencing psoriasis decreases the quality of life (QOL) and ends up in physical or mental imperfection when it is compared to patients suffering from other chronic diseases such as diabetes, depression, heart disease [44][2]. Moreover, according to data of global epidemiology, patient rate is 2 %–3 % (125 million people worldwide) of the world population [45][3]. Despite huge number of patients worldwide and huge amount of money being spent on drug research for psoriasis, there is no effective cure for the Psoriasis yet. Despite many efforts, Otezla is the first and only pill approved by FDA back in March 2014 [46][4]. Otezla is first selective inhibitor of phosphodiesterase 4 (PDE4) indicated for adults with active PsA. It is used to treat severities of plaque psoriasis ranging from mild, moderate, and severe [47][5]. At this moment, the treatments suggested for psoriasis patients are decided by the severity of the medical condition. To cure the mild psoriatic conditions topical therapies including corticosteroids and vitamin D analogues are used, whereas in worsening disease conditions, combination therapies are practiced. Combination therapies normally comprise of phototherapy along with systemic therapies (methotrexate, cyclosporine, acitretin). More advanced disease needs the oral-modifying antirheumatic drugs (DMARDs) or the TNF-α or 12/23 Interleukin (IL) inhibitors. Despite number of available treatment options, there is no effective treatment to fully eradicate the disease. Traditional way to find the drug/treatment that could eradicate the disease is called de-novo drug development. De-novo drug development takes a new drug molecule and performs all the preclinical validations [48][6], [49][7] to confirm the drug safety and efficacy in cell culture and animal models prior to use in human clinical trials. The whole process of de-novo drug development takes 12–17 years with $2∼$3 billion of investment and yet the total success rate is less than 10 % [50][8], [51][9]. This renders the de-novo drug development costly, time taking, and extremely risky process. Thus, a feasible alternative to de-novo drug development is drug repurposing which is the process of finding the alternative indications of already approved drugs. It reduces the time by 2–5 years and investment to $200∼$300 million [52][10], [53][11], [54][12]. A lot of work in general has been done in drug repurposing field. Many different techniques have also been used for drug repurposing for psoriasis. Advances in AI and ML have been witnessed for applications in psoriasis and other related conditions [55][13]. A recent Ensembl machine learning (ML) based study was published to predict the unknow drug-drug interactions (DDIs) for psoriasis [56][14]. >30 Sources were used for predicting the 37,611 unknown pairs of drugs. Another ML based work where Drug-disease relationships were modelled using the word-embedding of >20 million articles were presented in [57][15]. ML studies, despite being accurately predicting the DDIs, or drug-disease pairs are limited by lack of expansivity and inability to predict the clinical efficacy. Additionally, docking based study was done in [58][16] where 2000 FDA approved drugs were docked with 15 anti-psoriatic targets. Twelve drugs were prioritized based on docking score in the range of −12 to −11 kcal/mol. Molecular docking (MD) studies have been widely used in the drug discovery, but MD has some limitations such as incomplete information of binding pockets of the receptors, incomplete geometry, and lack of molecular flexibility etc. [59][17], [60][18]. Moreover, studies are also using the psoriasis associated genetic targets from GWAS for drug repurposing such as done in [61][19]. This study used the GWAS to find the 126 psoriasis related genes and using different tools 68 druggable proteins were identified. Small molecule (Pandal) targeting FDA approved POLI and interleukin 13 (IL 13) [62][20] were identified as potential therapeutic compound. Similar study is presented in [63][21] where, a framework was presented to repurpose the drugs for psoriasis using the clinical transcriptomic datasets derived from psoriasis, induced psoriasis-like and drug intervention samples. The molecular mechanisms in psoriasis were explored to find the repurposable compounds. The limitation of this is might the drug be agonist and the target gene are upregulated in medical condition and vice versa. Moreover, it is difficult to distinguish the causal genes in GWAS. To overcome these limitations, a disease-drug transcriptomic reversal approach can be used. Additionally, the previous drug repurposing techniques are using the animal models and in-vitro assays which also have two major limitations. First, these validation tools are sub-optimal representations of human disease and second these methods are time taking and costly [64][22]. Thus, here a drug-disease transcriptomic reversal-based drug repurposing techniques is proposed by integrating the disease gene signatures from clinical patients obtained from publicly available database (GEO) [65][23] and drug perturbation data to identify the potentially repurposable compounds. Moreover, the use of control and disease samples of a psoriasis disease signature true state of the disease is captured. The psoriasis signatures are then used to carry out database search in [66]clue.io portal [67][24] and drug compounds with highest negative values that could possibly reverse the disease. To validate the search results, peer reviewed literature, patents, and clinical database search was carried. Thus, this study represents a robust strategy to enable the psoriasis disease signature-based drug repurposing. Moreover, this method can be deployed to other disease of interest following the same series of steps as done in this study for psoriasis. The proposed mechanism can be generalized to any disease case, and it uses the databases and tools which are open source and easily accessible to smooth the drug repurposing process. 2. Materials and methods In this study, a disease-drug transcriptomic based drug repurposing framework for Psoriasis is presented. The differential gene expression signatures of Psoriasis are downloaded from GEO and for up and downregulated differential gene expression signatures, repurposable drugs that could reversal the expression signatures are found out. Drug expression signatures are taken directly from the LINCs associated [68]clue.io platform. A reversal score based on the Kolmogorov–Smirnov statistic (connectivity score) is generated for each disease-drug pair, with the idea that if the drug profile significantly reverses the psoriasis disease signature, it can stop the disease and be a potential therapy for a psoriasis. 2.1. Psoriasis gene expression signatures Three different psoriasis related gene expression datasets named [69]GSE13355, [70]GSE14905, and [71]GSE27628 were downloaded from GEO datasets as shown in [72]Fig. 1(a). [73]GSE13355 contains total 180 samples extracted from RNA taken punch biopsies of 58 psoriatic patients and 64 normal healthy controls presented in [74][25]. All 180 samples were run on Affymetrix HU133 Plus 2.0 microarrays containing >54,000 gene probes. Similarly, [75]GSE14905 consists of a total of 82 samples taken from 54 total subjects. In this dataset skin biopsy samples were collected from 21 normal healthy donors and 28 psoriasis patients with matched lesional and non-lesional tissue presented by the work done in [76][26] with the purpose of finding the potential therapeutic targets for Psoriasis. Finally, [77]GSE27628 dataset presented by [78][27] is a result of genome-wide expression profiling of five mouse models. This dataset is comprised of 34 samples in total. 17 samples were presented as control sample and other 17 samples represent disease samples. All the mentioned datasets files are provided as [79]Table S3 and [80]Table S4. Fig. 1. [81]Fig. 1 [82]Open in a new tab Drug repurposing strategy for Psoriasis. a) Gene expression data obtained from GEO with analysis being performed in GEO2R, b) followed by differential expression analysis and enrichment analysis. c) Database search based on the enriched gene list and d) finally performing validation. 2.2. Differential gene expression analysis All the three datasets were first analyzed using the on-site GEO2R ([83]Fig. 1(a)) tool to perform the differential gene expression (DEG) analysis. Across three datasets, all the normal samples were named as ‘normal’ and disease samples were name as ‘Psoriasis’. Processed files from GEO2R were downloaded for further analysis in Microsoft excel. The adjusted p-value setting of the GEO2R generated files were set below 0.05 while the Log2 fold change range was set between −1 and 1. After the initial analysis DGEs were ranked based on the Log2 fold change followed by the extraction of top 100 upregulated and downregulated genes ([84]Fig. 1(a)). The analyzed DEGs for [85]GSE13355 are mentioned in [86]Table S1, whereas for [87]GSE14905 and [88]GSE27628 top ranked list of DEGs are given in [89]Table S2 and [90]Table S3 respectively. (Adjusted p-value < 0.05, Log2 fold change value between −1 and 1, top ranked genes (Up regulated and down regulated)). 2.3. Pathway enrichment analysis Functional enrichment gene-set analysis was performed using the GSEA [91][28] software as shown in [92]Fig. 1(b). Genes were pre-ranked using the adjusted p-value and Log2 fold change value. Top 100 genes highly upregulated to downregulated in Psoriasis were fed to GSEA. Moreover, to perform the GSEA the hallmark gene sets (50) were retrieved directly from the MSigDB signature database [93][28]. This was followed by the KEGG pathway enrichment gene ontology (GO) terms identification of enriched biological process carried out using DAVID online tools ([94]Fig. 1(b)) and database [95][29]. Further the GO and pathway enrichment was also performed using the Enricher online tool [96][30]. 2.4. [97]Clue.io gene expression reversal and scoring Once the initial data extraction and data analysis is complete, next step is to perform the database search. Since the recent breakthroughs in technology have led to generation of high dimensional perturbational datasets. The huge data integrated across multiple assays, cells, and conditions of dose and treatment time require to be backed by sufficient computational expertise to be addressed. To meet such needs, connectivity map (CMap) [98][31], [99][32], [100][33] has developed a cloud-based software called CLUE for the analysis of perturbational datasets generated using gene expression. Here, CLUE has been used to find the small molecule drugs which could possibly reverse the gene expression signatures. This process was performed by first separating the top upregulated and top downregulated genes in Psoriasis ([101]Fig. 1(a-b)) and the “Query” tool of the clue platform was used to find positive and negative connections between gene expression signature of psoriasis genes and all the signatures in CMap to find the potential repurposable compounds ([102]Fig. 1(c)). The drug scoring algorithm used by CMap LINCS gene expression resources is referred to as connectivity score. It is a value ranging from −100 to +100 and it elucidates the connection between the disease associated queried signature and a drug perturbagen. Connectivity score usually contains three components: 1) False discovery rate (FDR) followed by 2) a nominal p-value, and 3) τ, which expresses the effect size of a mentioned enrichment score. τ is a dimensionless measure ranging from −100 to +100. Moreover, FDR adjust the p-value to use it for various hypothesis validation when the large number of comparisons are given. Similarly, p-value depicts the significance and similarity of the reference versus null distribution of the random queries. Next, the drugs were ranked, and top scoring drugs were taken for downstream analysis as shown in [103]Fig. 1(c). 2.5. Statistical analysis For initial analysis of the gene expression profiles, GEO2R, a R based tool was used and to find out the differential gene expression adjusted p-value and Log2 fold change ([104]Fig. 2) values were used [105][34]. Two different, psoriasis associated datasets identified with [106]GSE13355 and [107]GSE14905 were downloaded from the GEO database. [108]Fig. 2 shows the complete results obtained from the GEO2R tool. These results show the gene expression in psoriasis vs normal subjects as shown in [109]Fig. 2 (a & d). Similarly, clustering is also performed individually for both gene expression datasets with GEO identifiers as [110]GSE13355 and [111]GSE14905. Moreover, the gene expression datasets were clustered by GEO2R tool based on the psoriasis vs normal gene expression data and the resulting density of the expression in diseased vs normal samples are shown in [112]Fig. 2 (b & e). Additionally, the results of positive and negative data samples obtained from both gene expression datasets are given in [113]Fig. 2 (c & f). Later the genes, with Log2 fold change values in positive range were rendered as upregulated in Psoriasis while with negative values were called downregulated in Microsoft excel while setting the p value range to <0.05 and log2 fold change range of −1 and 1. Whereas the drugs because of query in “Clue” were ranked using the reversal scores. The more negative result for a drug, means the drug has higher potential to reverse the expression profiles. With this connections, top drugs with highest negative values were shortlisted for further analysis as shown in [114]Fig. 1(c). Fig. 2. [115]Fig. 2 [116]Open in a new tab GEO2R results for Psoriasis gene expressions. a) shows the psoriasis genes in normal vs psoriasis patients, b) shows the cluster of the Psoriasis vs normal individuals in the dataset with identifier [117]GSE13355, and c) depicts the total number of selected healthy vs disease samples. Similarly, d) shows the psoriasis genes in normal vs psoriasis patients for [118]GSE14905, e) clustering results of the total number of healthy vs psoriasis patients in the dataset, and finally f) presents the total number of control and psoriasis patient samples. 2.6. Validation of the ranked drugs To validate the ranked drugs, literature search was done. The peer reviewed journals, databases, and patents were mainly focused [119][11], [120][35], [121][36]. To avoid the bias, evidence was extended to positive and negative results for a given potential drug. To further elaborate, to handle the biases, search was carried out in two steps: i) Sources supporting the use of drug(s) for psoriasis and ii) Sources denying the use of drug(s) for psoriasis. According to the mentioned steps, if for any drug step (i) was true then it was termed as validated, if both steps were true then it was termed as not confirmed and were eliminated from further consideration, and finally if both steps were false for any drug, it was termed as potential candidate to be preclinically and clinically validated. In this the chances of false-positive or false-negative were minimized. Additionally, electronic health records and hospital databases were also traversed to find if the ranked drugs have already been used in psoriasis or not. 3. Results and discussions In this study, the developed repurposing pipeline was applied to psoriasis gene expression profiles to find the repurposable drugs that could potentially reverse the gene expressions and finally lead to inhibition of disease as shown in [122]Fig. 1. The repurposed drugs were later validated through literature and databases to confirm if the predicted potential drugs have already been used for the psoriasis or not. 3.1. Using gene expressions to find drug repurposing candidates To compute and find the disease associated gene-expression data we queried the public database (GEO) with disease-related terms. The resulting expressions that were shortlisted included the disease-associated gene expression changes [123][37], [124][38]. gene expression datasets named [125]GSE13355, [126]GSE14905, and [127]GSE27628 were downloaded from GEO and after performing p-test using GEO2R, top 100 genes both upregulated and downregulated were selected with adjusted p-value of < 0.05 and Log2FC value of −1 to 1. The analyzed DEGs for [128]GSE13355 are mentioned in [129]Table S3 and for [130]GSE14905 top ranked list of DEGs are given in [131]Table S4. Later these gene expressions were used to perform the database search in CMap database to find the compounds that could reverse these gene expression profiles. Top 150 repurposable drugs with highest potential to reverse the gene expression profiles were analyzed for [132]GSE13355 ([133]Table S1) and for [134]GSE14905 ([135]Table S2). Here, drugs with lowest score (−99) are referred to as high potential drugs that are expected to reverse the gene expression profiles and drugs with highest positive score (99) are referred to as the drugs which could promote the expression of the genes. 3.2. Gene set enrichment analysis We used GSEA (Gene set enrichment analysis) to perform the annotation of enriched hallmark pathways from each input signature as shown in [136]Fig. 3 (a). This was performed for both datasets ([137]GSE13355 and [138]GSE14905) and E2F, STAT5, STAT3, Interferon alpha, Interferon gamma, and inflammatory pathways were found upregulated ([139]Fig. 3 (b-g)). MTORC1 signaling, oxidative phosphorylation, P53, and spermatogenesis pathways were found downregulated. For the upregulated pathways, enrichment score is positive ([140]Fig. 3 (b-g)) and for the downregulated pathways the score is negative. Fig. 3. [141]Fig. 3 [142]Open in a new tab Gene set enrichment analysis. a) enrichment of pathways which shows the upregulated and downregulated pathways in psoriasis, b-g) the upregulated pathways including E2F, STAT5, STAT3, Inflammatory response, Interferon alpha, and Interferon gamma and their net enrichment scores respectively. 3.3. Literature based validation Literature search for Top 150 repurposable drugs from [143]GSE13355 ([144]Table 1) and [145]GSE14905 ([146]Table 2) was carried out in peer-reviewed journals and databases. The target was to find the any research study that have already use these drugs for psoriasis in pre-clinical or clinical setting. In case the drug is used, it is considered as a true hit and if there is no evidence for a drug in literature or database it means that it could be checked in pre-clinically (in-vitro and in-vivo) [147][39], [148][40] to check if the drug could work against psoriasis. As a result of this validation, it was found that 50/150 drugs from [149]GSE13355 and 37/150 for [150]GSE14905 have already been used for the psoriasis as shown in [151]Fig. 4. This confirms that, the approach used is potential and can correctly identify the drugs repurposable for psoriasis [152][41]. Among unvalidated drugs, top 10 drugs based on the criteria (Known indication and top score) are suggested as repurposable drug candidate for psoriasis ([153]Table 3 and [154]Fig. 5). [155]Fig. 5 shows the drug-target network of the suggested potential drugs repurposable for psoriasis and the network is created in the Cytoscape software after downloading the targets of the drugs from DrugBank. The target information of these drugs is obtained from DrugBank database. At the same time, the approach mentioned here could be extended to other disease of interest as well. Moreover, for the drugs that could not be confirmed from the literature survey, these should be first pre-clinically validated prior to use in humans. Finally, the limitations of the repurposing strategy are acknowledged such as missing values in gene expression profiles, uncertain conditions involved during carrying out experiments, and involved human error. Additionally, the mentioned limitations would be carried out as potential future work. Table 1. Literature based validated drugs shown to have anti-psoriasis activity. ([156]GSE13355). Drug Name Description Original indication Literature based validation Score 1 Tacedinaline HDAC inhibitor Lung Cancer Acne Vulgaris −93.05 2 GSK-3-inhibitor-II PKC inhibitor Neurodegenerative and psychiatric disorders Psoriasis −91.79 3 Cefpodoxime Bacterial cell wall synthesis inhibitor Various bacterial infections Skin and soft tissue infections −86.11 4 Alimemazine Histamine receptor agonist Allergic conditions Dermatology −83.47 5 Phylloquinone Vitamin K Psoriasis Psoriasis −79.42 6 Masitinib KIT inhibitor Tyrosine-kinase inhibitor Psoriasis −78.03 7 Dihydrexidine Dopamine receptor agonist Treatment of SPD – Trial Psoriasis −73.76 8 Sertraline Serotonin receptor antagonist Major depressive disorder Psoriasis −69.52 9 Domperidone Dopamine receptor antagonist Agent for dyspepsia, indigestion, epigastric pain, nausea, and vomiting Psoriasis −67.74 10 Norcyclobenzaprine Adrenergic receptor agonist Musculoskeletal conditions Dermatology −66.9 11 Sunitinib FLT3 inhibitor Advanced renal cell carcinoma (RCC) Psoriasis −66.25 12 Berbamine Calmodulin antagonist Various parasitic and fungal infections Psoriasis −65.16 13 Reserpine Vesicular monoamine transporter inhibitor Treatment of hypertension Psoriasis −63.34 14 Rimcazole Sigma receptor antagonist Antipsychotic Psoriasis −63.26 15 Ivermectin GABA receptor agonist Anti-parasite medication Psoriasis −61.68 16 Ramipril ACE inhibitor Mild to severe hypertension Psoriasis −60.09 17 Zalcitabine Nucleoside reverse transcriptase inhibitor Used to treat HIV Psoriasis −58.5 18 Serdemetan MDM inhibitor Treatment of Neoplasms – Trial Psoriasis −57.19 19 Gefitinib EGFR inhibitor Metastatic non-small cell lung cancer Psoriasis −56.89 20 Scriptaid HDAC inhibitor Enhance response of human tumor cells to radiation Psoriasis −55.84 21 Amlodipine Calcium channel blocker Hypertension and angina Psoriasis −55.04 22 Fluvoxamine Selective serotonin reuptake inhibitor (SSRI) Obsessive Compulsive Disorder (OCD) Psoriasis −55.02 23 Epothilone Microtubule inhibitor Treatment in various cancers Psoriasis −51.7 24 Triptolide RNA polymerase inhibitor HIV, Crohn's Disease, Intestinal Diseases - Trial Psoriasis −51.55 25 Hispidin PKC inhibitor Rheumatoid arthritis Psoriasis −49.69 26 Cyclosporin-a Calcineurin inhibitor Organ rejection in organ transplant Psoriasis −48.59 27 Ritonavir HIV protease inhibitor HIV infection Psoriasis −47.72 28 Mepacrine Cytokine production inhibitor Giardiasis and cutaneous leishmaniasis Psoriasis −46.06 29 Brimonidine Adrenergic receptor agonist Lowering intraocular pressure Dermatological −45.88 30 Norethisterone Progesterone receptor agonist Contraceptive when given as monotherapy Psoriasis −45.71 31 Pinocembrin CYP1B1 inhibitor – Psoriatic dermatitis −45.62 32 Carprofen Cyclooxygenase inhibitor Pain reliever in the treatment of joint pain and post-surgical pain Psoriatic-Like Dermatitis −45.6 33 Seneciphylline Cytochrome P450 inhibitor Component of Chinese medicine herb Psoriasis −44.78 34 Nelfinavir HIV protease inhibitor HIV infection Psoriasis −44.64 35 Acepromazine Dopamine receptor antagonist Antipsychotic agent Psoriasis −43.03 36 Bifonazole Sterol demethylase inhibitor Various topical fungal infections Psoriasis −42.7 37 Pramipexole Dopamine receptor agonist Parkinson’s disease Psoriasis −42.49 38 Rolitetracycline Bacterial 30S ribosomal subunit inhibitor Broad-spectrum tetracycline antibiotic Psoriasis −42.2 39 Flucloxacillin Bacterial cell wall synthesis inhibitor Bacterial infection by susceptible microorganisms Psoriasis −41.16 40 Ganciclovir DNA polymerase inhibitor Cytomegalovirus Psoriasis −40.02 41 Bupropion Dopamine uptake inhibitor Major depressive disorder (MDD) Psoriasis −39.97 42 Fraxidin Carbonic anhydrase inhibitor Antibacterial activity against Bacillus subtilis Psoriasis −39.27 43 Piperacillin Bacterial cell wall synthesis inhibitor Polymicrobial infections Psoriasis −38.49 44 Bis-tyrphostin EGFR inhibitor – Psoriasis −38.4 45 Ribavirin Antiviral Treatment of chronic Hepatitis C virus (HCV) Psoriasis −37.55 46 Sunitinib PLK inhibitor Renal cell carcinoma (RCC) Psoriasis −37.49 47 Testosterone Androgen receptor agonist Primary hypogonadism and hypogonadotropic hypogonadism Psoriasis −37.05 48 Atorvastatin HMGCR inhibitor Treatment of several types of dyslipidemias Psoriasis −36.48 49 Prostaglandin-e1 Prostanoid receptor agonist – Psoriasis −36.42 [157]Open in a new tab Table 2. Literature based validated drugs shown to have anti-psoriasis activity. ([158]GSE14905). Drug name Description Original indication Literature based validation Score 1 Parthenolide Nfkb pathway inhibitor Diagnostic of allergic contact dermatitis Psoriasis −97.18 2 Voriconazole Cytochrome P450 inhibitor Esophageal candidiasis, cadidemia Psoriasis −94.7 3 Diazepam Benzodiazepine receptor agonist Anxiety, seizures, alcohol withdrawal syndrome Psoriasis −93.82 4 Androstenedione Cytochrome p450 inhibitor Dietary supplement Psoriasis −89.91 5 Levetiracetam Calcium channel blocker Seizures in epileptic patients Psoriasis −89.15 6 Enzastaurin Pkc inhibitor Brain cancer, lymphoma (non-hodgkin's), and lung cancer. Psoriasis −88.53 7 Glimepiride Insulin secretagogue Type 2 diabetes Psoriasis −87.86 8 Quetiapine Dopamine receptor antagonist Schizophrenia and depression Psoriasis −87.63 9 Isoliquiritigenin Guanylate cyclase activator – Psoriasis −87.47 10 Pepstatin Aspartic protease inhibitor lung diseases, including hypersensitivity pneumonitis Psoriasis −86.82 11 Etomoxir Carnitine palmitoyltransferase inhibitor Inhibits complex i of the electron transport chain Psoriasis −85.3 12 Alfacalcidol Vitamin d receptor agonist Hypocalcemia, secondary hyperparathyroidism, and osteodystrophy Psoriasis −82.17 13 Benzylpenicillin Penicillin binding protein inhibitor Septicemia, meningitis, pericarditis, Psoriasis −78.79 14 Chelidonine Tubulin inhibitor – Psoriasis −77.06 15 Perindopril ACE inhibitor Hypertension, mild to moderate congestive heart failure Psoriasis −76.6 16 Liothyronine Thyroid hormone stimulant Hypothyroidism, nontoxic goiter, myxedema and myxedema coma Psoriasis −72.64 17 Tofacitinib Jak inhibitor Rheumatoid arthritis (ra), active psoriatic arthritis Psoriasis −72.22 18 Liquiritigenin Aromatase inhibitor – Psoriasis −69.64 19 Niacin Nad precursor with lipid lowering effects To reduce elevated tc, ldl-c, apo b and tg levels, and to increase hdl-c in patients with primary hyperlipidemia and mixed dyslipidemia Psoriasis −66.06 20 Taurodeoxycholic-acid Bile acid Cholestasis Psoriasis −64.91 21 Metanephrine Epinephrine metabolite Indicated in the emergency treatment of type i allergic reactions Psoriasis −64.83 22 Taurocholic-acid Bile acid – Skin inflammation −63.99 23 Retinol Retinoid receptor ligand Vitamin a deficiency Not good for psoriasis −62.7 24 Meloxicam Cyclooxygenase inhibitor Osteoarthritis, rheumatoid arthritis and moderate to severe pain Inflammatory conditions −62.14 25 Ganglioside Src activator Modulation of membrane proteins and ion channels, in cell signaling and in the communication among cells Proliferation of cultured keratinocytes −60.73 26 Dienestrol Estrogen receptor agonist Atrophic vaginitis and kraurosis vulvae Psoriasis, arthritis −58.84 27 Hypericin Tyrosine kinase inhibitor Inhibit the action of the enzyme dopamine β-hydroxylase, leading to increased dopamine levels Psoriasis and cutaneous t-cell lymphoma −56.14 28 Leu-enkephalin Opioid receptor agonist – Lesional psoriasis −56.05 29 Solanine Acetylcholinesterase inhibitor Fungicidal and pesticidal properties Psoriasis and inflammatory conditions −54.01 30 Pyrrolidine-dithiocarbamate Nfkb pathway inhibitor Chelation, induction of g1 phase cell cycle arrest Psoriasis −53.35 31 Phenolphthalein Indicator dye – Dermatology −52.75 32 Vinorelbine Tubulin inhibitor Non-small cell lung cancer (nsclc) Psoriatic skin lesions −52.3 33 Ofloxacin Bacterial dna gyrase inhibitor Treatment of infections (respiratory tract, kidney, skin, soft tissue, uti), urethral and cervical gonorrhoea. Infections of the skin −51.38 34 Hydroquinine Antiarrhythmic – Dark skin patches, and psoriasis −49.53 35 Prostaglandin Prostanoid receptor antagonist Platelet-activating-factor-inhibitor Psoriatic lesions −48.42 36 Phenamil Trpv antagonist Modulator of adipocyte differentiation and pparγ expression Psoriasis −46.02 [159]Open in a new tab Fig. 4. [160]Fig. 4 [161]Open in a new tab Depiction of number of predicted drugs followed by literature verified and not-verified drugs from both psoriasis expression datasets. Table 3. Top drugs repurposable for psoriasis. Drug name Description Original Indications Repurposable drug indication Score [162]GSE13355 1 Pyroxamide HDAC inhibitor Treatment of Leukemia Psoriasis −92.31 2 Droxinostat HDAC inhibitor Histone Deacetylase Inhibitor Psoriasis −91.57 3 Dolasetron Serotonin receptor antagonist Used in chemotherapy and postoperatively. Psoriasis −89.02 4 Ecopipam Dopamine receptor antagonist Treatment of Tourette's Syndrome Psoriasis −85.97 5 Nemonapride Dopamine receptor antagonist Treatment in gastroesophageal reflux disease Psoriasis −77.88 

 [163]GSE14905 6 Vecuronium Acetylcholine receptor antagonist Muscle relaxing agent and is used as an adjunct in general anesthesia. Psoriasis −93.31 7 Fosinopril ACE inhibitor Hypertension, congestive heart failure, slower the rate of progression of renal disease Psoriasis −90.19 8 Ziprasidone Dopamine receptor antagonist Schizophrenia Psoriasis −89.38 9 Securinine GABA receptor antagonist Neurological disorder Psoriasis −89.01 10 Carpindolol Adrenergic receptor antagonist Heart failure with reduced ejection fraction Psoriasis −88.58 [164]Open in a new tab Fig. 5. [165]Fig. 5 [166]Open in a new tab A network of drug-protein targets of the potential drugs repurposable for psoriasis. The drug targets used in the workflow are obtained from the DrugBank database. Moreover, the nodes in the green color represent the drugs whereas the nodes highlighted in the sky blue color represent the drug target proteins. (For interpretation of the references to