To address your questions regarding the pathway enrichment analysis from the article, here's a structured response: 1. **Tool Used for Pathway Enrichment Analysis:** - The tool used was GSEA (Gene Set Enrichment Analysis). 2. **Version Number of the Tool:** - Not described in the provided text. 3. **Gene Set Library Queried:** - The libraries queried included KEGG, GO (Gene Ontology), Reactome, and Hallmark gene sets. 4. **Background Gene List Defined:** - A background gene list was defined, consisting of genes expressed at an FPKM ≥ 1 in any of the samples being compared. 5. **Statistical Test Used for Enrichment Analysis:** - The enrichment analysis used a weighted Kolmogorov-Smirnov statistic. 6. **False Discovery Rate Correction:** - Yes, false discovery rate (FDR) correction was applied to control the number of false positives in the pathway enrichment analysis. This information can be collated into a table as follows: | Question | Answer | |----------|-----------------------------| | 1. Tool Used | GSEA | | 2. Version Number | Not described | | 3. Gene Set Library | KEGG, GO, Reactome, Hallmark | | 4. Background Gene List | Genes with FPKM ≥ 1 | | 5. Statistical Test | Weighted Kolmogorov-Smirnov | | 6. False Discovery Rate Correction | Yes | This table summarizes the key details about the pathway enrichment analysis as described in the article.