Okay, so I need to figure out how to answer these questions about the pathway enrichment analysis in this scientific article. The user has provided a detailed abstract and other sections, but not the full Methods section, which is where most of this information would be. Let me start by reading through what's given. First, the abstract mentions that they used nano-LC-MS/MS for proteomic analysis and identified proteins involved in "haemostasis," particularly TF. They also talk about functional enrichment analysis for GO terms like "cellular component" and "molecular function." So they're definitely doing some kind of pathway or gene set enrichment analysis. Now, looking at the discussion section, it mentions that functional enrichment analysis was done using GO domains and manually curated biological pathways. So they used both GO terms and other pathways, maybe Reactome or KEGG? But the text doesn't specify which ones beyond "haemostasis" and "platelet activation." The user's questions are about the tools, versions, gene sets, background lists, statistical tests, and FDR correction. Let me go through each: 1. **Tool used**: The article mentions functional enrichment analysis but doesn't name a specific tool like DAVID, Metascape, or g:Profiler. So I don't think it's described. 2. **Version number**: No version is provided in the text, so not described. 3. **Gene set library**: They mention GO terms and manually curated biological pathways. It doesn't specify if they used KEGG, Reactome, etc., just that they did GO and some other curated sets. 4. **Background gene list**: The article doesn't talk about this. Typically, you need a background list for over-representation analysis, but since it's not mentioned, I assume it's not described. 5. **Statistical test**: They don't specify which test was used—could be hypergeometric, Fisher's exact, etc.—so not described. 6. **FDR correction**: There's no mention of how they controlled for multiple comparisons or if they used FDR. So again, not described. I think the authors focused more on the biological findings rather than the detailed methodology of the pathway analysis. Without the Methods section, it's hard to get these specifics. | **Question** | **Answer** | |---------------------------------------------------|---------------------------------------------------------------------------| | 1. What tool was used for pathway enrichment analysis? | Not described. | | 2. Was a tool version number provided? | No version number was provided in the article. | | 3. What gene set library was queried (e.g., GO, KEGG, Reactome or other)? | Gene Ontology (GO) terms and manually curated biological pathways were used.| | 4. Was a background gene list defined for pathway enrichment analysis? | Not described. | | 5. What statistical test was used for enrichment analysis? | Not described. | | 6. Was false discovery rate correction used to control the number of false positives in the pathway enrichment analysis? | Not described. | **Summary**: The article does not provide detailed information about the specific tool, version, background gene list, statistical test, or FDR correction used for pathway enrichment analysis. It mentions the use of Gene Ontology (GO) terms and manually curated biological pathways but lacks specifics on other methodological details.