Here's a breakdown of the pathway enrichment analysis methods as described in the provided text, formatted for a table: | Question | Answer | |-----------------------------------------------|--------------------------------------| | 1. What tool was used for pathway enrichment analysis? | Not described | | 2. Was a tool version number provided? | Not described | | 3. What gene set library was queried? | GO (Gene Ontology) | | 4. Was a background gene list defined? | Not described | | 5. What statistical test was used? | Not described | | 6. Was false discovery rate correction used? | Yes, false discovery rate correction was used. | **Explanation based on the text:** * The text states "Functional enrichment analysis of the EV proteome for **GO** terms of the “cellular component” GO domain showed…." indicating use of Gene Ontology. * The text states "Functional enrichment analysis… revealed that… were strikingly up-regulated…". This indicates enrichment analysis was performed but doesn’t specify the statistical test. * The text states "false discovery rate correction was used to control the number of false positives", confirming FDR correction was applied. * The tool used, version number, background gene list and precise statistical test are *not* described in the provided text.