Here's an examination based on the details provided in your text regarding the methodology of pathway enrichment analysis: 1. **What tool was used for pathway enrichment analysis?** - The tool used for pathway enrichment analysis is the Kyoto Encyclopedia of Genes and Genomes (KEGG). 2. **Was a tool version number provided?** - No, the version number of the tool was not provided in the article. 3. **What gene set library was queried (e.g., GO, KEGG, Reactome or other)?** - The gene set library queried was KEGG. 4. **Was a background gene list defined for pathway enrichment analysis?** - A background gene list is not explicitly described in the article. 5. **What statistical test was used for enrichment analysis?** - The statistical significance of pathways was determined using P-values, but the specific statistical test used for enrichment analysis is not described. 6. **Was false discovery rate correction used to control the number of false positives in the pathway enrichment analysis?** - There is no mention of false discovery rate (FDR) correction being applied in the article. | Question | Answer | |----------|--------| | Tool used for pathway enrichment analysis | KEGG | | Tool version number provided | Not described | | Gene set library queried | KEGG | | Background gene list defined | Not described | | Statistical test used for enrichment analysis | P-values, specific test not described | | FDR correction used | Not mentioned | These findings are based on the information available in your text.