Thinking... We need to extract details about how pathway enrichment analysis was conducted from Methods section or relevant. The text snippet includes parts of Methods, but we have to parse the article. Let's search for "pathway enrichment", "IPA", "Ingenuity Pathway Analysis", etc. The snippet contains: "The Ingenuity Pathway Analysis (IPA) software was used to identify canonical signaling pathways and disease/biological function terms that were over-represented in each dataset." So tool: Ingenuity Pathway Analysis (IPA). Any other tool? Search for "Ingenuity" maybe other references to GSEA, DAVID. The text does not mention other tools. So only IPA. Now, "Were enrichment tool version(s) described?" The article may mention version. The text: "The Ingenuity Pathway Analysis (IPA) software (Qiagen) was used" but no version. Might have a version described somewhere else? Let's search: The article snippet may not show version. The text does not show version number. So Not described. "Which gene set libraries were used?" The article may mention canonical pathways, disease/biological function terms, maybe they used IPA's inbuilt libraries. In results tables, they mention KEGG, GO, Reactome? Let's read again. In Results, they mention: "Canonical pathways" from IPA. They refer to "biosynthesis of cell constituents", "cellular stress and death", etc. In methods, we saw: "Ingenuity Pathway Analysis (IPA) software was used to identify canonical signaling pathways and disease/biological function terms that were over-represented in each dataset." They mention "canonical signaling pathways" but not naming libraries. But IPA contains internal libraries. But the article may not specify which libraries they used: e.g., KEGG, Reactome, GO. They mention "canonical signaling pathways" but not naming the library. However, maybe they also used Gene Ontology, etc. Let's read: In results, they talk about "biosynthesis of cell constituents" "cellular stress and death" etc. They refer to "canonical pathways" that are over-represented. They mention "canonical signaling pathways" as part of IPA. So gene set libraries: Not described? They didn't specify using GO, KEGG, Reactome. However, IPA uses internal curated databases such as Ingenuity Pathway Knowledge Base, which is not a public library. But the article didn't specify which gene set libraries. So Not described. Now: custom background gene list: Not described. Statistical tests used: They mention "p < 0.05" but not test type. The article says: "A p < 0.05" for significance. But no test type. Did they mention "hypergeometric test" or "Fisher's exact test"? Not present. So Not described. Or maybe they used p-values from IPA; but not specified. So answer: Not described. Multiple testing correction: Not described. They only mention p<0.05 threshold, but no mention of FDR or Benjamini-Hochberg. So "Not described" or "No"? The instruction says only answer "Yes" if article explicitly mentions FDR, etc. So "Not described". Thus we create table. But we must list columns: "Tool", "Version", "Gene Set Libraries", "Custom Background", "Statistical Test", "Multiple Testing Correction". For each tool. Since only one tool: IPA. We must include all separate tools. If multiple tools, list all. Now we fill: Tool: Ingenuity Pathway Analysis (IPA) Version: Not described Gene Set Libraries: Not described (since not stated which libraries used). The article says "canonical signaling pathways" but not the specific library. So "Not described" Custom background gene list: Not described Statistical tests: Not described (maybe hypergeometric but not specified). So "Not described" Multiple testing correction: Not described Thus produce table: | Tool | Version | Gene Set Libraries | Custom Background | Statistical Tests | Multiple Testing Correction | | IPA | Not described | Not described | No | Not described | Not described | But wait: We should answer "Which gene set libraries were used? Identify all gene set libraries or databases the authors queried (e.g., GO, KEGG, Reactome, MSigDB, etc.). Only include what's actually stated in the article." If not stated, write "Not described". Hence, yes. Thus output table. Check that we didn't miss any other tool used: They used microarray analysis using SurePrint G3 Human Gene Expression Microarray 8x60k. But that's preprocessing. The enrichment tool: IPA. Might also have used "Ingenuity Pathway Analysis (IPA) software" and "Ingenuity Knowledge Base" but still a tool. They used "canonical signaling pathways" which is a term within IPA. They didn't mention "Gene Ontology", etc. Thus answer. But we should also include "Ingenuity Pathway Analysis" as a tool. Thus final answer. ...done thinking. | Question | Answer | |---|---| | **Tool(s) used for pathway enrichment analysis** | Ingenuity Pathway Analysis (IPA) | | **Enrichment tool version(s) described** | Not described | | **Gene set libraries used** | Not described | | **Custom or specific background gene list used** | Not described | | **Statistical test(s) used in enrichment analysis** | Not described | | **Multiple testing correction / FDR applied** | Not described |