Here are the answers to the questions: 1. What tool was used for pathway enrichment analysis? The tools used for pathway enrichment analysis were DAVID (Database for Annotation, Visualization and Integrated Discovery) and WebGestalt 2017. 2. Was a tool version number provided? Yes, the version numbers of the tools were provided: DAVID version 6.8 and WebGestalt 2017. 3. What gene set library was queried (eg: GO, KEGG, Reactome or other)? The gene set libraries queried were Gene Ontology (GO) and KEGG pathways. 4. Was a background gene list defined for pathway enrichment analysis? Yes, the background gene list used was the Bos taurus genome. 5. What statistical test was used for enrichment analysis? Not described. 6. Was false discovery rate correction used to control the number of false positives in the pathway enrichment analysis? Yes, a False Discovery Rate (FDR) of 5% was used to correct for multiple testing.