1. **What tool was used for pathway enrichment analysis?** - DAVID (Database for Annotation, Visualization and Integrated Discovery) version 6.8 and WebGestalt 2017. 2. **Was a tool version number provided?** - Yes, DAVID v6.8 and WebGestalt 2017 were specified. 3. **What gene set library was queried (e.g., GO, KEGG, Reactome or other)?** - Gene Ontology (GO) terms (BP, CC, MF) and KEGG pathways were used. 4. **Was a background gene list defined for pathway enrichment analysis?** - Yes, the Bos taurus genome was used as the background. 5. **What statistical test was used for enrichment analysis?** - The specific statistical tests are not described in detail; however, p-values and false discovery rates (FDR) were mentioned. 6. **Was false discovery rate correction used to control the number of false positives in the pathway enrichment analysis?** - Yes, FDR correction with a threshold of 5% was applied.