Based on the provided text, here is an examination of how pathway enrichment analysis was conducted in this scientific article: 1. **Tool Used for Pathway Enrichment Analysis:** - The tool used for Gene Ontology (GO) enrichment analysis is the GOseq R package. - For Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, KOBAS software was utilized. 2. **Tool Version Number Provided:** - The version number for the DESeq R package is provided as v1.18.0. - No specific versions are mentioned for GOseq or KOBAS in the text provided. 3. **Gene Set Library Queried:** - For GO enrichment, a Gene Ontology (GO) gene set library was queried. - For pathway analysis, the KEGG pathways were queried using KOBAS software. 4. **Background Gene List Defined for Pathway Enrichment Analysis:** - The text does not explicitly mention the definition of a background gene list for pathway enrichment analysis. It is generally implied that the entire reference genome or transcriptome could be used as a background, but this detail is not described in the excerpt. 5. **Statistical Test Used for Enrichment Analysis:** - For GO enrichment, a statistical test using corrected P-values (P-value ≤ 0.5) was employed. - The specific statistical test for KOBAS pathway analysis is not detailed, but typically, enrichment tools like these use hypergeometric or similar tests. 6. **False Discovery Rate Correction Used:** - For DEG identification using the DESeq package, Benjamini and Hochberg’s method was used to adjust P-values for false discovery rate (FDR) control. - For GO enrichment analysis, corrected P-values were utilized, indicating some form of FDR correction. Here's how this information can be collated into a table format: | Question | Details | |----------|---------| | Tool Used for Pathway Enrichment Analysis | GOseq R package (GO), KOBAS software (KEGG) | | Tool Version Number Provided | DESeq v1.18.0; no specific versions mentioned for GOseq or KOBAS | | Gene Set Library Queried | GO (Gene Ontology), KEGG pathways | | Background Gene List Defined | Not described | | Statistical Test Used for Enrichment Analysis | Corrected P-value ≤ 0.5 for GO; not specified for KEGG | | False Discovery Rate Correction Used | Benjamini and Hochberg’s method for DESeq, corrected P-values used in GO | If more details are required or specific sections of the article need further examination, additional context from the full text would be needed.