Here's a breakdown of the pathway/gene enrichment analysis methods, based on the provided text, presented in a format suitable for a table: **1. What tool was used for pathway enrichment analysis?** * DAVID (Database for Annotation, Visualization and Integrated Discovery) **2. Was a tool version number provided?** * Yes, version 6.8 is specified. **3. What gene set library was queried (eg: GO, KEGG, Reactome or other)?** * Gene Ontology (GO) terms were queried. **4. Was a background gene list defined for pathway enrichment analysis?** * Yes. A list of 10,919 genes reported as expressed in bovine skeletal muscle (from the EMBL-EBI Expression Atlas) was used as the background gene list. **5. What statistical test was used for enrichment analysis?** * Not explicitly described in the provided text. The text only states that significance of enriched terms was adjusted. **6. Was false discovery rate correction used to control the number of false positives in the pathway enrichment analysis?** * Yes, Benjamini-Hochberg p-value correction was used to adjust for multiple testing and control for false positives. --- **Table Format:** | Question | Answer | |---------------------------------------------------|-----------------------------------------------| | 1. Tool used for pathway enrichment analysis? | DAVID | | 2. Tool version number? | 6.8 | | 3. Gene set library queried? | Gene Ontology (GO) | | 4. Was a background gene list defined? | Yes (10,919 genes from EMBL-EBI Expression Atlas) | | 5. Statistical test used for enrichment analysis? | Not described | | 6. False discovery rate correction used? | Yes (Benjamini-Hochberg) |