Certainly! Here is an examination of how pathway enrichment analysis was conducted based on the provided article information: 1. **Tool Used for Pathway Enrichment Analysis**: - The DAVID Bioinformatic Resources 6.8 server was used. 2. **Tool Version Number Provided**: - Yes, version number 6.8 is specified. 3. **Gene Set Library Queried**: - Gene Ontology (GO) terms were queried as indicated by the presence of GO term enrichment in the additional files and results sections. 4. **Background Gene List Defined**: - A background gene list was defined using a set of 10,919 genes reported as expressed in bovine skeletal muscle from the EMBL-EBI Expression Atlas (as noted in Additional file 12). 5. **Statistical Test Used for Enrichment Analysis**: - The Benjamini-Hochberg p-value correction method was used to adjust significance levels during enrichment analysis. 6. **False Discovery Rate Correction**: - Yes, false discovery rate correction was applied using the Benjamini-Hochberg method. This summary can be collated into a table for easier reference: | Question | Answer | |----------|--------| | Tool Used for Pathway Enrichment Analysis | DAVID Bioinformatic Resources 6.8 server | | Tool Version Number Provided | Yes, version 6.8 | | Gene Set Library Queried | GO (Gene Ontology) | | Background Gene List Defined | Yes, from EMBL-EBI Expression Atlas (10,919 genes) | | Statistical Test Used for Enrichment Analysis | Benjamini-Hochberg p-value correction | | False Discovery Rate Correction Used | Yes | This information was extracted and interpreted based on the provided details. If further clarification is needed or additional sections of the article are referenced, adjustments may be necessary.