Alright, I need to figure out how to answer these six questions about the pathway enrichment analysis from the provided scientific article. The user wants me to examine the Methods section and other relevant parts for details. Let's go through each question one by one. 1. **What tool was used for pathway enrichment analysis?** - I'll start by skimming the Methods section of the article. The Methods usually mention the tools or software used in analyses like pathway enrichment. - Looking through, I don't see any mentions of specific tools like DAVID, Metascape, or Enrichr. The authors discuss their approach to data analysis but don't specify a particular tool for pathway enrichment. 2. **Was a tool version number provided?** - Since the first point didn't find any tool mentioned, it's unlikely that a version number was provided either. Without knowing the tool, version details aren't possible. 3. **What gene set library was queried (e.g., GO, KEGG, Reactome or other)?** - Again, checking the Methods and Results sections for mentions of gene sets. The article talks about biological pathways and processes but doesn't specify which databases were used for enrichment analysis. They might be using internal resources or standard databases, but it's not stated. 4. **Was a background gene list defined for pathway enrichment analysis?** - This is about whether the authors used a specific set of genes as the background or universe in their analysis. The article doesn't discuss this aspect explicitly, so I can't confirm if they did or didn't define such a list. 5. **What statistical test was used for enrichment analysis?** - Statistical methods are crucial here. However, the Methods section doesn't detail which tests were applied for pathway enrichment. Common tests include hypergeometric tests or others, but without specifics, I can't determine what they used. 6. **Was false discovery rate correction used to control the number of false positives in the pathway enrichment analysis?** - The article mentions statistical verification and confidence levels when discussing biological phenomena, but there's no direct mention of FDR correction specifically for pathway enrichment. They might have used it, but it's not described. After reviewing all sections, it seems the authors didn't provide detailed information on their pathway enrichment methodology in the article. Therefore, most answers will be "Not described." | Question | Answer | |-------------------------------------------------------------------------|----------------------| | What tool was used for pathway enrichment analysis? | Not described | | Was a tool version number provided? | Not described | | What gene set library was queried (e.g., GO, KEGG, Reactome or other)? | Not described | | Was a background gene list defined for pathway enrichment analysis? | Not described | | What statistical test was used for enrichment analysis? | Not described | | Was false discovery rate correction used to control the number of false positives in the pathway enrichment analysis? | Not described |