Alright, I need to figure out how to answer these questions about the pathway enrichment analysis in the provided scientific article. Let's go through each question one by one. 1. **What tool was used for pathway enrichment analysis?** - The article mentions that they performed statistical overrepresentation tests of top miR-SNP hits. Looking at the Results section, they specifically mention using tools like PANTHER or maybe another method, but in this case, it seems they used PANTHER for the pathway analysis. 2. **Was a tool version number provided?** - Skimming through the Methods and Results sections, I don't see any mention of specific version numbers for the tools used. The focus is more on the results than the exact software versions. 3. **What gene set library was queried (e.g., GO, KEGG, Reactome or other)?** - In the article, under the miR-SNP analysis, they reference pathways like the MHC protein complex and IFN-γ signaling, which are part of Gene Ontology (GO) terms. So, it's safe to say they used GO for some of their analyses. Additionally, they might have used Reactome since one pathway mentioned is specifically from Reactome. 4. **Was a background gene list defined for pathway enrichment analysis?** - The article doesn't explicitly state whether a specific background gene list was used. Typically, in such analyses, the default is to use all genes on the array or in the genome unless specified otherwise, but since they didn't mention it, I can't assume. 5. **What statistical test was used for enrichment analysis?** - They mention using overrepresentation tests, which usually involve hypergeometric tests or similar methods. The exact test isn't detailed beyond "statistical overrepresentation." 6. **Was false discovery rate correction used to control the number of false positives in the pathway enrichment analysis?** - The article doesn't discuss FDR correction explicitly. They mention P-values but not whether they adjusted them using FDR. Putting this all together, I can answer each question based on the information provided and what's inferred from standard practices when details aren't explicit. | Question | Answer | |-------------------------------------------------------------------------|---------------------------------------------------------------------------------------| | What tool was used for pathway enrichment analysis? | PANTHER | | Was a tool version number provided? | Not described | | What gene set library was queried (e.g., GO, KEGG, Reactome or other)? | Gene Ontology (GO) and Reactome | | Was a background gene list defined for pathway enrichment analysis? | Not described | | What statistical test was used for enrichment analysis? | Statistical overrepresentation tests (likely hypergeometric tests) | | Was false discovery rate correction used to control the number of false positives in the pathway enrichment analysis? | Not described |