Based on the information provided from the scientific article, here is an examination of how pathway enrichment analysis was conducted: 1. **What tool was used for pathway enrichment analysis?** - The tool used for pathway enrichment analysis was PANTHER (Protein ANalysis THrough Evolutionary Relationships). 2. **Was a tool version number provided?** - No specific version number of the PANTHER tool is mentioned in the text. 3. **What gene set library was queried (e.g., GO, KEGG, Reactome or other)?** - The gene sets queried included those from Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG). 4. **Was a background gene list defined for pathway enrichment analysis?** - Yes, the entire genome was used as a reference set for GO terms. 5. **What statistical test was used for enrichment analysis?** - The specific statistical tests are not described in detail in the article provided. 6. **Was false discovery rate correction used to control the number of false positives in the pathway enrichment analysis?** - Yes, a false discovery rate (FDR) cutoff of 0.05 or less was applied for significance. Here is how this information can be tabulated: | Question | Answer | |----------|--------| | Tool Used | PANTHER | | Tool Version Number | Not described | | Gene Set Library Queried | GO, KEGG | | Background Gene List Defined | Yes (entire genome for GO) | | Statistical Test Used | Not described | | FDR Correction Used | Yes (cutoff of 0.05 or less) | This table summarizes the pathway enrichment analysis details as described in the article.