Okay, let's start by looking at the user's query. They want me to examine how pathway enrichment analysis was conducted in the provided scientific article, focusing on the Methods section and possibly other sections. The user has listed six specific questions to answer, and each answer should be separate so they can be collated into a table. If any details aren't provided, I should note that as "not described." First, I need to go through the Methods section of the article provided. The user included the entire Methods section, so I can refer to that directly. Let's break down each question one by one. Question 1: What tool was used for pathway enrichment analysis? Looking through the Methods section, specifically under "4.6. Proteome Data Analysis," the authors mention that they used multiple public databases including KEGG. However, the actual tool for pathway enrichment analysis might be mentioned here. The text states, "To analyze the potential functions of the proteins, we first reannotated the rice proteins... mapped to... KEGG databases." Then, when discussing the calculation of p-values and Q-values, they mention using Fisher’s exact test and the R package "q-value." But the specific tool for pathway enrichment might be the KEGG database itself, but the analysis tool isn't explicitly named. Wait, in the Methods section under "4.6," after mapping to databases, they mention using Fisher’s exact test and the R package "q-value." However, the actual pathway analysis tool (like DAVID, GSEA, etc.) isn't specified. The answer might be that the tool isn't explicitly stated, but they used KEGG for the database. The question is about the tool, not the database. So maybe the answer is "not described" since the specific tool isn't mentioned, just the databases. Question 2: Was a tool version number provided? In the same section, the authors mention using "Mascot v2.3.02 (Matrix Science)" for protein identification, but for pathway enrichment, there's no mention of a tool version. The answer here is "no" or "not described." Question 3: What gene set library was queried (e.g., GO, KEGG, Reactome or other)? In the Methods section under "4.6," they state that proteins were mapped to "KEGG databases." So the answer here is KEGG. Question 4: Was a background gene list defined for pathway enrichment analysis? The text says, "Using all the proteins as background..." So yes, the background gene list was defined as all the proteins in the rice database. The answer is "yes." Question 5: What statistical test was used for enrichment analysis? The authors mention, "The p-values were calculated by Fisher’s exact test..." So the statistical test is Fisher's exact test. Question 6: Was false discovery rate correction used to control the number of false positives in the pathway enrichment analysis? Yes, they state that the Q-values were calculated using the R package "q-value," which is used for FDR correction. The answer is "yes." Now, compiling all these answers into the required format. Each question is answered separately, and if a detail isn't provided, it's marked as "not described." Let me double-check each point to ensure accuracy. 1. **What tool was used for pathway enrichment analysis?** The article does not explicitly state the specific tool used for pathway enrichment analysis. It mentions mapping proteins to the KEGG database but does not specify a dedicated enrichment analysis tool (e.g., DAVID, GSEA, etc.). 2. **Was a tool version number provided?** Not described. 3. **What gene set library was queried (e.g., GO, KEGG, Reactome or other)?** **KEGG** (Kyoto Encyclopedia of Genes and Genomes). 4. **Was a background gene list defined for pathway enrichment analysis?** Yes. The authors used **all proteins in the rice database** as the background gene list. 5. **What statistical test was used for enrichment analysis?** **Fisher’s exact test** (as described in the Methods section under "Proteome Data Analysis"). 6. **Was false discovery rate correction used to control the number of false positives in the pathway enrichment analysis?** Yes. The **Q-values** (false discovery rate, FDR) were calculated using the R package "q-value," with a threshold of **FDR < 0.05**. --- **Summary Table** | Question | Answer | |----------|--------| | 1. Tool for pathway enrichment analysis | Not described | | 2. Tool version number | Not described | | 3. Gene set library queried | KEGG | | 4. Background gene list defined | Yes (all rice proteins) | | 5. Statistical test | Fisher’s exact test | | 6. FDR correction | Yes (Q-values with FDR < 0.05) |