Based on the provided article, here is the information regarding the pathway enrichment analysis: 1. **What tool was used for pathway enrichment analysis?** - ToppFun was used for pathway enrichment analysis. 2. **Was a tool version number provided?** - No, the version number of ToppFun was not provided. 3. **What gene set library was queried (e.g., GO, KEGG, Reactome or other)?** - Multiple databases were queried: Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database, and the Molecular Signatures Database (MSigDB). 4. **Was a background gene list defined for pathway enrichment analysis?** - Yes, a background gene set was described as containing all genes present in the datasets that were analyzed. 5. **What statistical test was used for enrichment analysis?** - The article does not specify which statistical test was used for enrichment analysis; thus, it is noted as "not described." 6. **Was false discovery rate correction used to control the number of false positives in the pathway enrichment analysis?** - Yes, a p-value cutoff of <0.05 with a Benjamini-Hochberg FDR threshold of 0.05 was applied, indicating that false discovery rate (FDR) correction was used. Here is how this information can be tabulated: | Question | Answer | |----------|--------| | What tool was used for pathway enrichment analysis? | ToppFun | | Was a tool version number provided? | No | | What gene set library was queried? | GO, KEGG, MSigDB | | Was a background gene list defined for pathway enrichment analysis? | Yes | | What statistical test was used for enrichment analysis? | Not described | | Was false discovery rate correction used to control the number of false positives in the pathway enrichment analysis? | Yes (Benjamini-Hochberg FDR threshold of 0.05) |