Okay, so I need to figure out how the pathway enrichment analysis was conducted in this article. Let me start by looking through the provided sections. The user mentioned that the Methods section might have the info, but since the actual Methods section isn't included here, I'll have to piece it together from the Results and Discussion sections, as well as other parts like the references and tables. First, the question is about the tool used for pathway enrichment. In the Results section under the GO functional enrichment analysis, they mention using ToppGene suite's ToppFun. For example, in the footnotes of tables 5 and 6 (though not visible here, the text refers to ToppFun). The authors state that the results were obtained through ToppFun, part of the ToppGene suite. So the tool is ToppFun. Next, the version number. The text just mentions ToppFun without specifying the version. The references cited (like [94]28 and others) might have version info, but since I can't access the references, the article itself doesn't state the version. So probably, the version isn't provided. For the gene set library, the GO terms are mentioned in the GO functional enrichment. The Results section says "Gene Ontology (GO) functional enrichment analysis" and lists terms like cellular locomotion, mobility, etc., which are GO categories. They also mention miRNA families like miR-29, which might relate to other libraries, but the main one here is GO. The tool ToppFun uses GO, so that's the library. Background gene list: The authors analyzed 82 genes related to wound healing. The Methods probably mention that they selected these genes first, then performed enrichment. The background might be the entire genome or the 82 genes. But in enrichment analysis, background is usually all possible genes. However, the article says they analyzed differentially expressed genes (30 out of 82), so maybe the background is the 82 genes? Or the entire transcriptome? The text says they used the differentially expressed genes (significantly deregulated) for the analysis. Since ToppFun typically uses a background, but the paper doesn't specify, so it might not be described here. Statistical test: The Results mention FDR (false discovery rate) correction. The question is what test was used before correction. Typically, GO enrichment uses hypergeometric test or Fisher's exact. The authors mention statistical significance with FDR, so the test underlying might be hypergeometric. But the exact test isn't stated explicitly. The paper says "functional enrichment analysis predicted..." but not the test. Since ToppFun uses hypergeometric, that's likely, but need to confirm from the text. The text doesn't specify the test, so maybe it's not described. FDR correction: The Results do mention FDR (e.g., "FDR: False discovery rate"), so yes, they used FDR correction. The question is whether they applied it. The answer is yes. Putting this together: 1. Tool: ToppFun (part of ToppGene suite) 2. Version: Not provided 3. Gene set library: GO (Gene Ontology) 4. Background gene list: Not explicitly described in the text provided. The analysis used the 30 significantly deregulated genes, but background might be the entire set of genes analyzed (82?) or the genome. Since not mentioned, answer is "not described" 5. Statistical test: Not explicitly stated, but likely hypergeometric as part of ToppFun's process, but since the text doesn't say, answer is "not described" 6. FDR correction: Yes, mentioned as part of the analysis (they refer to FDR in the tables and footnotes) 1. **Tool used for pathway enrichment analysis?** ToppFun (part of the ToppGene suite). 2. **Was a tool version number provided?** Not described. 3. **Gene set library queried?** Gene Ontology (GO). 4. **Was a background gene list defined?** Not described. The analysis focused on 30 significantly deregulated wound-healing-related genes, but the background gene list (e.g., entire transcriptome or a specific set) was not explicitly specified. 5. **Statistical test used?** Not explicitly stated. While enrichment analyses typically use tests like the hypergeometric test or Fisher’s exact test, the article does not specify the statistical test applied. 6. **Was false discovery rate (FDR) correction used?** Yes. The article mentions FDR correction in the context of multiple testing (e.g., "FDR: False discovery rate" in footnotes of tables). --- ### Summary Table: | Question | Answer | |--------------------------------------------------------------------------|-------------------------------------------------------------------------| | 1. Tool used for pathway enrichment analysis? | ToppFun (ToppGene suite) | | 2. Tool version number provided? | No | | 3. Gene set library queried (e.g., GO, KEGG, Reactome)? | Gene Ontology (GO) | | 4. Background gene list defined? | Not described | | 5. Statistical test used for enrichment analysis? | Not described (likely hypergeometric, but not explicitly stated) | | 6. FDR correction used for false positives? | Yes (FDR correction mentioned in the text) |