Thinking... We need to examine the article to find all information about pathway enrichment analysis. The Methods section mentions "The NIH Database for Annotation, Visualization and Integrated Discovery (DAVID) was used to perform gene ontological category enrichment analysis using Homo sapiens as background, and default options and annotation categories." So tool: DAVID. Version not described? It didn't specify version number. Gene set libraries used: Gene Ontology categories (GO). Perhaps also KEGG or other? The article says "gene ontological category enrichment analysis" which refers to GO. It doesn't mention KEGG, Reactome, MSigDB. So gene set library: GO (Gene Ontology). Did they use a custom or specific background gene list? They used "Homo sapiens as background". Not a custom list, so answer "No". Statistical test used: The tool DAVID uses Fisher's exact test or hypergeometric test? But it's not stated. The article says "gene ontological category enrichment analysis" but does not mention the statistical test. So "Not described". Was multiple testing correction applied? The article doesn't mention FDR for enrichment. It says "Using DAVID Functional Annotation Clustering" and table shows "Adjusted P-value" for each cluster, which suggests multiple testing correction was applied by DAVID. But does the article explicitly mention FDR? The table says "Adjusted P-value". They didn't say FDR. The default of DAVID is to use a p-value based on Fisher's exact test and correct for multiple testing using Benjamini-Hochberg. But the article didn't mention FDR. So "No". We must output table with 6 columns. Table columns: Tool | Version described? | Gene set libraries | Custom/background list? | Statistical test | Multiple testing correction? But question: "Was multiple testing correction / FDR applied to enrichment analysis?" Only answer Yes if explicitly mentions. The article didn't mention it. So No. Also "Does the article describe using a custom or specific background gene list for enrichment analysis?" Options Yes, No, Not described. They used Homo sapiens as background, not custom, so No. Check supplementary: Table 3 shows DAVID Functional Annotation Clustering with Enrichment score, Representative category, Count, Adjusted P-value. So they had Adjusted P-value, but that is for enrichment categories? Yes. But still the method didn't mention correction. The Adjusted P-value is likely BH. But they didn't mention method. So "No". Also results mention "immune response- and interferon signaling-related pathways were statistically overrepresented". That is part of enrichment analysis. The only tool is DAVID. Let's check if they used GSEA or other: Search article. Methods mention "Gene ontological category enrichment analysis" and "DAVID". No other. Results: Table 3 shows DAVID Functional Annotation Clustering. No mention of other tools. Thus table: Question 1: tool(s) used for pathway enrichment analysis: DAVID Question 2: Were enrichment tool version(s) described?: Not described Question 3: Which gene set libraries were used? GO Question 4: Custom/background list? No Question 5: Statistical test(s) used: Not described But the question says "Only include tests linked to enrichment." If test not described, answer Not described. Question 6: Was multiple testing correction applied to enrichment analysis? They say "using Homo sapiens as background, and default options and annotation categories." They don't mention FDR. So No. Thus output table with 6 columns. But we need to list all tools or software packages used (e.g., DAVID, GSEA, Enrichr). There's only DAVID. No version. Gene set libraries: GO. Custom background: No. Statistical tests: Not described. Multiple testing correction: Not described (No). Let's produce table. But the instructions: "If a detail is not present, write 'Not described'." For question 2: "Were enrichment tool version(s) described?" So answer: Not described. For question 4: "Does the article describe using a custom or specific background gene list for enrichment analysis? Your options include Yes, No or Not described." We said No. For question 5: "What statistical test(s) were used in enrichment analysis?" state name(s) of the statistical test(s) used for enrichment analysis (e.g., hypergeometric test, Fisher's exact test). Only include tests linked to enrichment. Only include tests linked to enrichment. The article didn't specify test. So "Not described". Now produce table format. Let's create a table with columns: - Tool - Version - Gene Set Libraries - Custom background list? (Yes/No/Not described) - Statistical test(s) - Multiple testing correction Row: DAVID | Not described | Gene Ontology | No | Not described | No Also maybe there is another tool: "DAVID Functional Annotation Clustering" used. That's still DAVID. No other tool. Therefore final answer. ...done thinking. | Question | Answer | |---|---| | **What tool was used for pathway enrichment analysis?** | DAVID | | **Were enrichment tool version(s) described?** | Not described | | **Which gene set libraries were used?** | Gene Ontology (GO) | | **Does the article describe using a custom or specific background gene list for enrichment analysis?** | No | | **What statistical test(s) were used in enrichment analysis?** | Not described | | **Was multiple testing correction / FDR applied to enrichment analysis?** | No | *All details are taken directly from the article; any missing information is reported as “Not described.”*