Thinking... We need to extract the pathway enrichment analysis details. From the Methods section, I see "Gene enrichment analysis" subheading. Let's parse it. They performed tissue restricted gene enrichment and also a second analysis using DAVID. They mention "available GO terms for molecular function, cellular component and biological process" using DAVID Bioinformatic Resources 6.8 server. So tool: DAVID. Also, they mention "second analysis performed using DAVID Bioinformatic Resources 6.8 server". So same tool. There's also "hypergeometric test available in the Apache Commons Mathematics Library for JAVA" used in enrichment. They also used the Benjamini-Hochberg p-value correction. Let's list all tools: DAVID Bioinformatic Resources 6.8. Did they use any other? I think only DAVID. Also they mention "Apache Commons Mathematics Library for JAVA" for hypergeometric test. But that is not an enrichment tool; it's a library. But maybe it's used for statistical test. But question: "Which tool was used for pathway enrichment analysis?: List all tools or software packages used (e.g., DAVID, GSEA, Enrichr, etc.) that are explicitly mentioned in the article for enrichment analysis." So we list DAVID. Are there any other? They also mention "R package limma" used to visualize gene overlapping, not enrichment. "IGraph" for network. "Prosite" "SignalP" for protein predictions. But not for enrichment. So only DAVID. "Were enrichment tool version(s) described?" Yes: 6.8 server. "Which gene set libraries were used?" They used GO terms for 7 phenotypes. They mention GO terms for molecular function, cellular component and biological process. They didn't mention KEGG or Reactome. So gene set library: Gene Ontology (GO). Also maybe "DAVID" uses GO, but they didn't mention KEGG or Reactome. So only GO. They mention 105, 89, 85 etc GO terms. So library: GO. "Does the article describe using a custom or specific background gene list for enrichment analysis?" They used background gene list of 10,919 genes expressed in bovine skeletal muscle from Expression Atlas. For tissue restricted gene enrichment, they filtered genes expressed in bovine skeletal muscle. That is the background. Also for DAVID-based enrichment, they used "default DAVID background list". So yes, custom background for first enrichment. So answer: Yes. "What statistical test(s) were used in enrichment analysis?" They used hypergeometric test. They mention "hypergeometric test available in the Apache Commons Mathematics Library for JAVA". So test: hypergeometric test. (Maybe also Fisher exact test is same but not mentioned). So answer: hypergeometric test. "Was multiple testing correction / FDR applied to enrichment analysis?" They say "Benjamini-Hochberg p-value correction" was performed for each pathway by trait. So Yes. Now produce table with columns: Tool, Version, Gene set library, Background gene list used? (Yes/No/Not described), Statistical test, Multiple testing correction? Provide. Also maybe "DAVID Bioinformatic Resources 6.8 server" is the tool. They mention version 6.8. So we provide that. No other enrichment tools. Let's produce table. Also, note "The available GO terms for molecular function, cellular component and biological process were included in the analysis using a GO term estimation for the background list in DAVID Bioinformatic Resources 6.8 server." So gene set library: GO. Also they mention "available GO terms" for each trait: 105, 89, 85, 92, 81, 86, 95 GO terms. So that indicates GO library. Also "The gene lists with 1714, 1636, 1632, 1753, 1753, 1666, and 1684 genes for WBSF, marbling, cooking loss, tenderness, juiciness, connective tissue, and flavor, respectively, were used." So maybe they used the gene sets from the background. But we don't need to detail. Now produce table with 6 rows? They ask 6 questions, produce table with each column: what they ask. Let's produce: | Question | Answer | But they ask to provide output in table format. They ask: "Provide output in table format." So we need to provide a table with columns: (1) Tool used for pathway enrichment analysis, (2) Enrichment tool version(s), (3) Gene set libraries used, (4) Custom background gene list used? (Yes/No/Not described), (5) Statistical tests used, (6) Multiple testing correction applied. So we can create one row per tool maybe. But we only have one tool: DAVID Bioinformatic Resources 6.8. So we can create a table with columns: "Tool", "Version", "Gene set library", "Custom background gene list", "Statistical test", "Multiple testing correction". So the row: "DAVID Bioinformatic Resources" | "6.8" | "GO" | "Yes" | "Hypergeometric test" | "Yes". Also we might need to include "Apache Commons Mathematics Library" but that's not enrichment tool. They might not want to list. But question: "Which statistical test(s) were used in enrichment analysis?" They used hypergeometric test. So yes. We may mention "Benjamini-Hochberg" correction. So "Yes". Ok. Let's produce table. ...done thinking. | Question | Answer | |---|---| | **What tool was used for pathway enrichment analysis?** | DAVID Bioinformatic Resources | | **Were enrichment tool version(s) described?** | 6.8 | | **Which gene set libraries were used?** | Gene Ontology (GO) – molecular function, cellular component, biological process | | **Did the article describe using a custom or specific background gene list for enrichment analysis?** | Yes | | **What statistical test(s) were used in enrichment analysis?** | Hypergeometric test | | **Was multiple testing correction / FDR applied to enrichment analysis?** | Yes (Benjamini‑Hochberg) |