FastQCFastQC Report
Wed 15 Sep 2021
3166-POD1_S266_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename3166-POD1_S266_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34765455
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACTAGAATCTCGGGT584670.1681755639326452TruSeq Adapter, Index 4 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACTAGAATCTCGGTT448260.12893833835915566TruSeq Adapter, Index 4 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)