FastQCFastQC Report
Wed 15 Sep 2021
PG053-POD1_S32_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePG053-POD1_S32_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38661083
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGGTACATCTCGTAT1900480.49157443416678215TruSeq Adapter, Index 3 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGGTACATCGCGTAT1116520.2887968761764899TruSeq Adapter, Index 3 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)