FastQCFastQC Report
Wed 15 Sep 2021
PG3248-POD1_S72_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePG3248-POD1_S72_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40816144
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC1079340.2644394825733661No Hit
CTCGGCTTTGACAGAGTGCAAGACGATGACTTGCAAAATGTCGCAGCTGG689820.16900665579776472No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA503140.12326985126277484No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC458760.11239670263805418No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)